umkhiqizo

Incazelo emfushane:

I-antigen ye-SEM imbozwe endaweni yokuhlola ye-nitrocellulose membrane yamapheshana, futhi i-SEM antibody ibhalwe ngegolide eliyi-colloid. Ngesikhathi sokuhlolwa, i-colloid yegolide ebhalwe ukuthi i-antibody embozwe emgqeni iya phambili eduze kolwelwesi, futhi umugqa obomvu uzovela lapho i-antibody ihlangana ne-antigen emgqeni wokuhlola; uma i-SEM kusampula ingaphezu komkhawulo wokutholwa, i-antibody izosabela nama-antigen kusampula futhi ngeke ihlangabezane ne-antigen kulayini wokuhlola, ngaleyo ndlela ngeke kube khona umugqa obomvu emugqeni wokuhlola.