Le khithi isuselwe kubuchwepheshe bokuncintisana obungaqondile be-immunochromatography, lapho i-Malachite Green kusampula iqhudelana ngegolide eline-colloid elibhalwe ukuthi i-antibody ne-Malachite Green ehlanganisa i-antigen ethathwe kulayini wokuhlola. Umphumela wokuhlolwa ungabukwa ngeso lenyama.