Ngesikhathi sangemuva esandayo sezinkinga zokuphepha kokudla, uhlobo olusha lwekhithi lokuhlola ngokususelwa kuI-enzyme-ixhumeke i-Imunosorbent Assay (ELISA)kancane kancane iba yithuluzi elibalulekile emkhakheni wokuhlolwa kokuphepha kokudla. Akugcini nje ngokunikeza izindlela eziqondile nezisebenzayo zokuqapha kwekhwalithi yokudla kepha futhi kwakha umugqa wokuvikela oqinile wokuphepha kokudla kwabathengi.
Umgomo we-ELISA Test Kit ulele ekusebenziseni ukusabela okuhlanganisiwe phakathi kwe-antigen ne-antibody ngobuningi ekuboneni okuqukethwe kwezinto eziqondiwe ekudleni okuhlosiwe. Inqubo yayo yokusebenza ilula futhi inokucaciswa okuphezulu nokuzwela, okunika amandla ukukhonjwa okunembile nokukalwa kwezinto eziyingozi ekudleni, njenge-aflatoxin, ochratoxin a, futhiT-2 ubuthi.
Ngokwezinqubo ezithile zokusebenza kokusebenza, i-ELISA Test Kit ngokuvamile ifaka lezi zinyathelo ezilandelayo:
1
2. Isengezo sesampula: Isixazululo sesampula esisetshenzisiwe sengezwe emithonjeni eqokiwe epuletini ye-ELISA, nento ehambelana kahle nento ehambelana nento okufanele ihlolwe.
I-3. I-Incubation: Ipuleti le-ELISA elinamasampula angeziwe lifakwe emazingeni okushisa afanele isikhathi eside ukuvumela ukubopha okugcwele phakathi kwama-antigens nama-antibodies.
I-4. Ukugeza: Ngemuva kokufakwa, isixazululo sokuwasha kusetshenziselwa ukususa ama-antigns noma ama-antibodies angabusi abondies, kunciphisa ukuphazanyiswa kokubopha okungenangqondo.
5.Ukungezwa kwe-substrate kanye nokuthuthukiswa kombala: Isixazululo se-substrate sengezwe kumthombo ngamunye, kanye ne-enzyme ku-enzyme-elibhalwe nge-antibody catalyzes the substrate substrate ukuthuthukisa umbala, wakhe umkhiqizo onemibala.
I-6. Ukulinganiswa: Inani lokumunca umkhiqizo onemibala emthonjeni ngamunye lilinganiswa kusetshenziswa amathuluzi afana nomfundi e-Elisa. Okuqukethwe kwento okufanele kuhlolwe kubhalwe kususelwa kujika elijwayelekile.
Kunamacala amaningi wohlelo lokusebenza lwe-Elisa Test Kits ekuhlolweni kokuphepha kokudla. Isibonelo, ngesikhathi sokuhlolwa kokuphepha kokudla okujwayelekile nokuhlolwa kwesampula, iziphathimandla ezilawulwa yimakethe zisebenzise ikhithi lokuhlola le-ELISA ukuze zithole ngokushesha amazinga e-Aflatoxin B1 kumafutha e-peanut akhiqizwa yi-peanatoxin kawoyela. Izindlela ezifanele zenhlawulo zathathwa ngokushesha, ngempumelelo ukuvimbela into eyingozi kusuka kubathengi abasengozini.
Ngaphezu kwalokho, ngenxa yokusebenza kwayo kalula ukusebenza, ukunemba, kanye nokwethenjwa, i-ELISA Test Kit isetshenziswa kabanzi ekuhlolweni kokuphepha kokudla okuhlukahlukene okufana nemikhiqizo yasemanzini, imikhiqizo yenyama nemikhiqizo yobisi. Akuvamisile nje kuphela isikhathi esiningi sokutholwa futhi kuthuthukise ukusebenza kahle kepha futhi kunikeza nokusekelwa okunamandla kwezobuchwepheshe kwabaphathi abalawulayo ukuqinisa ukubhekwa kwemakethe yokudla.
Ngokuthuthuka okuqhubekayo kobuchwepheshe kanye nokuqaphela okwandayo kokuphepha kokudla phakathi kwabantu, ama-Elisa Test Kits azodlala indima ebaluleke kakhulu emkhakheni wokuhlolwa kokuphepha kokudla. Esikhathini esizayo, sibheke phambili ekuqubukeni okuqhubekayo kwezinto ezintsha zobuchwepheshe, ngokuhlangene okukhuthaza ukukhula okunamandla komkhakha wokuphepha kokudla futhi kunikeze isiqinisekiso esiqinile sokuphepha kokudla kwabathengi.
Isikhathi sePosi: Dece-12-2024