I-China Immunoaffinity Colounts ye-Zearalenone Detection Factory kanye nabakhiqizi

I-Imunoaffinity Colounts ye-Zearalenone Detection efakiwe

I-Imunoaffinity Colounts yokutholwa kwe-zearalenone

Incazelo emfushane:

Amakholomu we-Kwinbon Zearalenone asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ELISA Test Kit. Kungaba ukuhlolwa kwenani le-AFB1 ukuze uthole okusanhlamvu kanye nemikhiqizo yokusanhlamvu, uviniga, imikhiqizo yesoso, utshwala, ama-soya, izinto zokudlwengulwa kanye nemikhiqizo eqediwe, njll.

Thumela i-imeyili kithi Izinkomba zokusetshenziswa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukucaciswa komkhiqizo

Ikati cha.	Kh00304z
Izakhiwo	IngomaneIndlu yezwelenaneukuhlola
Indawo yemvelaphi	Beijing, China
I gama le-brand	Kwinbon
Usayizi weyunithi	Izivivinyo ezingama-25 ebhokisini ngalinye
Uhlelo lwesampula	Imikhiqizo emibi nemikhiqizo yokusanhlamvu, u-soy sauce, imikhiqizo yesoso, imikhiqizo yesoso, utshwala, uSoya, u-rapeed kanye nemifino yemifino, njll.
Ukubeka	2-30 ℃
Ishalofu-impilo	Izinyanga eziyi-12
Ukuhambisa	Igumbi le-Tempepere

Izinsizakusebenza & ama-reagents adingekayo

Izinsizakusebenza

Reagents

Izinsizakusebenza

---- homogenizer ---- vortex mixer

---- Isampula ibhodlela ---- Ukulinganisa isilinda: 10ml, 100ml

---- I-Quictitative Filter Paper / Centrifuge ---- Ukuhlaziya Ibhalansi (I-Intactance: 0.01G)

---- I-PIPETTE: 10ML ---- I-Indeltor: 20ml

---- I-Volumetric Flask: 250ML ---- I-Rubber Pipette Bulb

---- I-micropipette: 100-1000l ---- glanwork 50ml

---- Ukuhlunga kwe-Microfiber (Whatman, 934-AH, φ11cm, 1.5um Circle)

Reagents

---- Methanol (AR)

---- acetic acid (a acectice acid (ac)

---- sodium chloride (nacl, ar)

---- Amanzi aDeionized

Izinzuzo Zomkhiqizo

I-Zearalenone (Zen), eyaziwa nangokuthi i-RAL ne-F-2 Mycotoxin, i-esrogenic metabolite enamandla ekhiqizwayo. Kuzinzile okushisa futhi kutholakala emhlabeni wonke ezitshalweni eziningi zokusanhlamvu, njengommbila, ibhali, ama-oats, ukolweni, ilayisi kanye ne-sorghum.

I-Zearalenone ingubuthi oyinhloko obopha ama-estrogen receptors, abangele ukuzala, ukukhipha isisu noma ezinye izinkinga zokuzala, ikakhulukazi ezingulubeni.

I-Wikipedia incoma izindlela ezilandelayo zokutholwa;

I-chromatography ye-chromatography encane ye-chromatography,
I-Enzyme-Ixhumanise I-Imunosorbent Assay
Immunoaffinity coloorescence
I-Imunoaffinity Course Phezulu Ukusebenza Kwe-Chromatography

I-Kwinbon Inmmunoaffinity Colounts ingeyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ngokwahlukana, ukuhlanzwa noma ukuhlaziywa okuthile kweZearalenone. Imvamisa amakholomu amabhoni ahlanganiswe ne-HPLC.

Ukuhlaziywa kwe-HPLC ngobuningi bobuthi fungal kuyindlela evuthiwe yokutholwa. Kokubili phambili futhi ukubuyisa isigaba se-chromatography kuyasebenza. Isigaba sokubuyela emuva i-HPLC singokomnotho, kulula ukusebenza, futhi sinobuthi obuphansi be-solvent. Ubuthi obuningi buncibilika ngezigaba ze-polar mobile bese uhlukaniswa yikholofini ye-chromatography engekho, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi obuningi fungal kusampula yobisi. Ama-Uplic ahlanganisiwe ahlanganisiwe ayasetshenziswa kancane kancane, ngamamojula aphezulu wengcindezelo kanye nosayizi omncane kanye namakholomu we-chromatography amakholomu, anciphise ukusebenza kwesampula, futhi athole ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu we-Kwinbon Zearalenone angabamba ama-molecule ahlosiwe esimweni esihlanzekile kakhulu. Futhi amakholomu we-kWinbon ahamba ngokushesha, ama-Easy asebenze. Manje kushesha futhi kusetshenziswa kabanzi insimu yokuphakelayo nenhlamvu ye-Mycotoxins Desetion.