I-China Immunoaffinity columns ye-Zearalenone ukuthola imboni nabakhiqizi

Amakholomu e-Immunoaffinity wokutholwa kwe-Zearalenone

Incazelo emfushane:

Amakholomu e-Kwinbon Zearalenone asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA. Kungaba ukuhlolwa okulinganiselwe kwe-AFB1 kokusanhlamvu nemikhiqizo yokusanhlamvu, isoso yesoya, uviniga, imikhiqizo yesoso, utshwala, ubhontshisi wesoya, amafutha embewu yokudlwengula kanye nemifino, ukondla izinto ezingavuthiwe nemikhiqizo eqediwe, njll.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00304Z
Izakhiwo	NgobaZearalenoneukuhlola
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Okusanhlamvu nemikhiqizo yokusanhlamvu, isoso ye-soy, uviniga, imikhiqizo yesoso, utshwala, ubhontshisi wesoya, amafutha e-rapeseed nemifino, ukondla izinto ezingavuthiwe nemikhiqizo eqediwe, njll.
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

I-Zearalenone (ZEN), eyaziwa nangokuthi i-RAL kanye ne-F-2 mycotoxin, iyi-metabolite enamandla ye-estrogenic ekhiqizwayo. Iyakwazi ukumelana nokushisa futhi itholakala emhlabeni wonke ezitshalweni eziningi zokusanhlamvu, njengommbila, ibhali, i-oats, ukolweni, irayisi namabele.

I-Zearalenone iwubuthi obuyinhloko obubophezela kuma-estrogen receptors, okubangela ukungazali, ukukhipha isisu noma ezinye izinkinga zokuzalanisa, ikakhulukazi ezingulubeni.

I-Wikipedia incoma izindlela zokuhlonza ezilandelayo;

I-chromatography yongqimba oluncane,
Ukuhlolwa kwe-immunosorbent okuhlotshaniswa ne-enzyme
Ikholomu ye-Immunoaffinity fluorescence
Ikholomu ye-Immunoaffinity yokusebenza okuphezulu kwe-chromatography ye-liquid

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ukuhlukanisa, ukuhlanzwa noma ukuhlaziya okuthile kwe-Zearalenone. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Zearalenone angakwazi ukubamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.