Amakholomu e-China Immunoaffinity wefekthri yokuthola i-Vomitoxin nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Vomitoxin Isithombe Esifakiwe

Amakholomu e-Immunoaffinity ukuze kutholwe i-Vomitoxin

Incazelo emfushane:

Amakholomu e-Kwinbon Vomitoxin asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA.Kungaba ukuhlolwa okulinganiselwe kwe-AFB1 kokusanhlamvu, amantongomane nemikhiqizo yawo, amafutha emifino namafutha, imikhiqizo yamantongomane, isoso yesoya, uviniga, umuthi wamaShayina, izinongo kanye netiye.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00203Z
Izakhiwo	Okokuhlola i-Vomitoxin
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Okusanhlamvu, amantongomane nemikhiqizo yawo, amafutha emifino namafutha, imikhiqizo yamantongomane, isoso yesoya, uviniga, umuthi wamaShayina, izinongo netiye
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

I-Vomitoxin, eyaziwa nangokuthi i-deoxynivalenol (DON), iyi-epoxy-sesquiterpenoid. Kwenzeka kakhulu okusanhlamvu okunjengokolweni, ibhali, oats, rye, nommbila, futhi kancane erayisini, amabele, kanye ne-triticale, okubangela ukuhlanza kanye ne-anorexia ezilwaneni futhi okungase kuholele esicanucanu nokuhlanza kubantu.

I-Wikipedia incoma izindlela zokuhlonza ezilandelayo;

I-chromatography yongqimba oluncane,
Ukuhlolwa kwe-immunosorbent okuhlotshaniswa ne-enzyme
Ikholomu ye-Immunoaffinity fluorescence
Ikholomu ye-Immunoaffinity yokusebenza okuphezulu kwe-chromatography ye-liquid

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ukuhlukanisa, ukuhlanzwa noma ukuhlaziya okuthile kwe-Vomitoxin. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Vomitoxin angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.