Amakholomu e-China Immunoaffinity we-T-2 imboni yokutholwa kwe-mycotoxin nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-T-2 mycotoxin

Incazelo emfushane:

Amakholomu e-Kwinbon T-2 mycotoxin asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA.Kungaba ukuhlolwa kwenani le-T-2 mycotoxin yezinhlamvu nemikhiqizo yokusanhlamvu, isoso yesoya, uviniga, imikhiqizo yesoso, utshwala, ubhontshisi wesoya, amafutha embewu ye-rapeseed kanye nemifino, ukondla izinto ezingavuthiwe nemikhiqizo eqediwe, njll.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH01301Z
Izakhiwo	NgobaT-2 mycotoxinukuhlola
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Okusanhlamvu nemikhiqizo yokusanhlamvu, isoso yesoya, uviniga, imikhiqizo yesoso, utshwala, njll.
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

I-T-2 mycotoxin iwumkhiqizo ovela ngokwemvelo wesikhunta we-Fusarium spp. ukhunta futhi inobuthi kubantu nakwezinye izilwane. Isimo somtholampilo esiwudalayo i-alimentary toxic aleukia kanye nenqwaba yezimpawu ezihlobene nezitho ezihlukahlukene njengesikhumba, indlela yokuphefumula, nesisu. Itholakala ku-T-2 mycotoxins okusanhlamvu okungcolile, ukudla nokudla.

I-Kwinbon Inmmunoaffinity Columns iyingxenye yesithathu, isebenzisa i-chromatography ewuketshezi ukuhlukanisa, ukuhlanzwa noma ukuhlaziya okuthile kwe-T-2 mycotoxin. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon T-2 mycotoxin angabamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.