I-China Immunoaffinity Colounts ye-Ochratoxin Ifektri Yabakhiqizi nabakhiqizi

I-Imunoaffinity Colounts ye-Ochratoxin I-Detectived Image

Amakholomu we-immunoaffinity ye-Ochratoxin okutholwe

Incazelo emfushane:

I-Kwinbon Ochratoxin Amakholomu asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ELISA Test Kit. Kungaba ukuhlolwa kwe-Ochratoxin a for the okusanhlamvu kanye nemikhiqizo yokusanhlamvu, u-soy sauce, uviniga, imikhiqizo yesoso, utshwala, cocoa kanye nekhofi ebilisiwe, njll.

Thumela i-imeyili kithi Izinkomba zokusetshenziswa

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Ukucaciswa komkhiqizo

Ikati cha.	Kh00404z
Izakhiwo	IngomaneOchratoxin a ukuhlola
Indawo yemvelaphi	Beijing, China
I gama le-brand	Kwinbon
Usayizi weyunithi	Izivivinyo ezingama-25 ebhokisini ngalinye
Uhlelo lwesampula	GIzimvula kanye nemikhiqizo yokusanhlamvu, isoso soya, uviniga, imikhiqizo ye-sauce, utshwala, cocoa kanye nekhofi ebilisiwe, njll.
Ukubeka	2-30 ℃
Ishalofu-impilo	Izinyanga eziyi-12
Ukuhambisa	Igumbi le-Tempepere

Izinsizakusebenza & ama-reagents adingekayo

Izinsizakusebenza

Reagents

Izinsizakusebenza

---- homogenizer ---- vortex mixer

---- Isampula ibhodlela ---- Ukulinganisa isilinda: 10ml, 100ml

---- I-Quictitative Filter Paper / Centrifuge ---- Ukuhlaziya Ibhalansi (I-Intactance: 0.01G)

---- I-PIPETTE: 10ML ---- I-Indeltor: 20ml

---- I-Volumetric Flask: 250ML ---- I-Rubber Pipette Bulb

---- I-micropipette: 100-1000l ---- glanwork 50ml

---- Ukuhlunga kwe-Microfiber (Whatman, 934-AH, φ11cm, 1.5um Circle)

Reagents

---- Methanol (AR)

---- acetic acid (a acectice acid (ac)

---- sodium chloride (nacl, ar)

---- Amanzi aDeionized

Izinzuzo Zomkhiqizo

Njengoba kwaziwa i-Mycotoxin, OChratoxin A (OCRATOXIN A (OTA) ekhiqizwa yizinhlobo ezimbalwa fungal kufaka phakathi i-Aspergillus Ochraceus, A. Carbonarius, A. Niger nePenicillium Verrucosum. U-Ota ubangela ama-nephrotoxicicity kanye nama-renal tumors ezinhlobonhlobo zezilwane; Kodwa-ke, imiphumela yezempilo yabantu ayibonakali kahle.

I-Kwinbon Inmmunoaffinity Colounts ingeyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ngokwahlukana, ukuhlanza noma ukuhlaziywa okuthile kwe-Ochratoxin A. Imvamisa amakholomu ahlanganiswe ne-HPLC.

Ukuhlaziywa kwe-HPLC ngobuningi bobuthi fungal kuyindlela evuthiwe yokutholwa. Kokubili phambili futhi ukubuyisa isigaba se-chromatography kuyasebenza. Isigaba sokubuyela emuva i-HPLC singokomnotho, kulula ukusebenza, futhi sinobuthi obuphansi be-solvent. Ubuthi obuningi buncibilika ngezigaba ze-polar mobile bese uhlukaniswa yikholofini ye-chromatography engekho, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi obuningi fungal kusampula yobisi. Ama-Uplic ahlanganisiwe ahlanganisiwe ayasetshenziswa kancane kancane, ngamamojula aphezulu wengcindezelo kanye nosayizi omncane kanye namakholomu we-chromatography amakholomu, anciphise ukusebenza kwesampula, futhi athole ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, i-kWinbon Ochratoxin Amakholomu angabamba ama-molecule ahlosiwe esimweni esihlanzekile kakhulu. Futhi amakholomu we-kWinbon ahamba ngokushesha, ama-Easy asebenze. Manje kushesha futhi kusetshenziswa kabanzi insimu yokuphakelayo nenhlamvu ye-Mycotoxins Desetion.