Amakholomu e-China Immunoaffinity we-Ochratoxin Imboni yokutholwa nabakhiqizi

Amakholomu e-Immunoaffinity wokutholwa kwe-Ochratoxin A

Incazelo emfushane:

Amakholomu e-Kwinbon Ochratoxin A asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA. Kungaba ukuhlolwa okulinganiselwe kwe-Ochratoxin A kokusanhlamvu nemikhiqizo yokusanhlamvu, isoso yesoya, uviniga, imikhiqizo yesoso, utshwala, uKhokho nekhofi eligazingiwe, njll.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00404Z
Izakhiwo	NgobaOchratoxin A ukuhlola
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Gizimvula nemikhiqizo yokusanhlamvu, isoso yesoya, uviniga, imikhiqizo yesoso, utshwala, ukhokho nekhofi eligazingiwe, njll.
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

Njengoba yaziwa nge-mycotoxin, i-Ochratoxin A (OTA) ekhiqizwa izinhlobo ezimbalwa zesikhunta ezihlanganisa i-Aspergillus ochraceus, A. carbonarius, A. niger kanye ne-Penicillium verrucosum. I-OTA ibangela i-nephrotoxicity kanye nezimila zezinso ezinhlobonhlobo zezilwane; kodwa-ke, imiphumela yempilo yabantu ayibonakali kahle.

I-Kwinbon Inmmunoaffinity Columns iyingxenye yesithathu, isebenzisa i-chromatography ewuketshezi ukuhlukanisa, ukuhlanzwa noma ukuhlaziya okuthile kwe-Ochratoxin A. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Ochratoxin A angakwazi ukubamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.