Amakholomu e-China Immunoaffinity wefekthri yokuthola i-Fumonisin nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Fumonisin Isithombe Esifakiwe

Amakholomu e-Immunoaffinity ukuze kutholwe i-Fumonisin

Incazelo emfushane:

Amakholomu e-Kwinbon Fumonisin asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA. Ilungele okusanhlamvu, ukudla, imithi yamaShayina, njll futhi ithuthukisa ubumsulwa bamasampula.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH01001Z
Izakhiwo	NgobaI-Fumonisinukuhlola
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Okuphakelayo, okusanhlamvu, okusanhlamvu nezinongo
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

I-Fomonisin(FB) iwuhlobo lwama-metabolites ancibilikayo emanzini akhiqizwa kusuka kusikhunta esine-toxigenic. Ingcolisa kakhulu okusanhlamvu nemikhiqizo yazo, futhi inobuthi obunamandla kanye nobungozi obunamandla be-carcinogenicity emfuyweni ethile, futhi isibe enye indawo yocwaningo ngemuva kwe-aflatoxin. Kungabangela umonakalo omkhulu kubantu nasezilwaneni ukuthi le nto ivezwe kabanzi emmbila nakweminye imikhiqizo yezolimo.Muva nje i-fumonisin isiphenduke enye yezinto ezimbi kakhulu ezingcolisa umhlaba.Ucwaningo mayelana nokuvinjelwa nokulawulwa kwe-fumonisin seluphenduke isizinda socwaningo emhlabeni wonke. umhlaba.

I-Wikipedia incoma izindlela zokuhlonza ezilandelayo;

I-chromatography yongqimba oluncane,
Ukuhlolwa kwe-immunosorbent okuhlotshaniswa ne-enzyme
Ikholomu ye-Immunoaffinity fluorescence
Ikholomu ye-Immunoaffinity yokusebenza okuphezulu kwe-chromatography ye-liquid

I-Kwinbon Inmmunoaffinity Columns iyindlela yesithathu, isebenzisa i-chromatography ewuketshezi ukuhlukanisa, ukuhlanzwa noma ukuhlaziya okuthile kwe-Fumonisin. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Fumonisin angakwazi ukubamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.