Amakholomu e-China Immunoaffinity we-Aflatoxin Total ifektri nabakhiqizi

Amakholomu e-Immunoaffinity we-Aflatoxin Inani Lesithombe Esifakiwe

Amakholomu e-Immunoaffinity we-Aflatoxin Total

Incazelo emfushane:

Amakholomu e-AFT asetshenziswa ngokuhlanganisa nekhithi yokuhlola ye-HPLC, LC-MS, ELISA.

Kungaba ukuhlolwa kwenani i-AFB1, AFB2, AFG1, AFG2. Ilungele okusanhlamvu, ukudla, imithi yamaShayina, njll futhi ithuthukisa ubumsulwa bamasampula.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH01102Z
Izakhiwo	Ukuhlola Ingqikithi ye-Aflatoxin
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Okuphakelayo, okusanhlamvu, okusanhlamvu nezinongo
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

Amakholomu e-Kwinbon Inmmunoaffinity asebenzisa i-chromatography ewuketshezi ukuze ahlukanise, ahlanze noma ahlaziye okuthile kwe-Aflatoxin Total. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.
I-monoclonal antibody ngokumelene ne-Aflatoxin Total ixhunywe nemidiya ehlanganisayo kukholamu. Ama-Mycotoxins kusampula ayakhishwa, ayahlungwa futhi ahlanjululwe. Yenza isixazululo sokukhipha isampula sidlule kukholamu ye-Aflatoxin Total. I-Aflatoxin (B1, B2, G1, G2) insalela ihlanganiswa ne-antibody ngokwehlukana kukholamu, isixazululo sokuwasha sisusa ukungcola okungahlanganisiwe. Okokugcina, ukusebenzisa i-methyl alcohol ukuze kukhishwe i-Aflatoxin B1, i-Aflatoxin B2, i-Aflatoxin G1, i-Aflatoxin G2.
Ngokucaciswa okuphezulu, amakholomu e-Kwinbon AFT angakwazi ukubamba ama-molecule aqondiwe esimweni esihlanzeke kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha nangokubanzi endaweni yokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.