Amakholomu e-China Immunoaffinity wefekthri yokuthola i-Aflatoxin M1 nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Aflatoxin M1 Isithombe Esifakiwe

Amakholomu e-Immunoaffinity okutholwa kwe-Aflatoxin M1

Incazelo emfushane:

Amakholomu e-Kwinbon Aflatoxin M1 asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA.

Kungaba ukuhlolwa kwenani i-AFM1 yobisi oluwuketshezi, iyogathi, impushana yobisi, ukudla okukhethekile okudliwayo, ukhilimu noshizi.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00902Z
Izakhiwo	Okokuhlola i-Aflatoxin M1
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Lubisi lwe-iquid, iyogathi, impushana yobisi, ukudla okukhethekile okudliwayo, ukhilimu noshizi
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

----- Amanzi ahlanjululwe

Izinzuzo zomkhiqizo

Amakholomu e-Kwinbon Inmmunoaffinity asebenzisa i-chromatography ewuketshezi ukuze ahlukanise, ahlanze noma ahlaziye okuthile kwe-Aflatoxin M1. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kwe-HPLC ngobuningi bobuthi besikhunta kuyindlela yokuthola evuthiwe. Kokubili i-chromatography yesigaba sangaphambili nesangemuva kuyasebenza. I-HPLC yesigaba sangemuva iyonga imali, kulula ukuyisebenzisa, futhi inobuthi obuphansi be-solvent. Ubuthi obuningi buyancibilika ezigabeni ezihambayo ze-polar bese buhlukaniswa ngamakholomu e-non-polar chromatography, okuhlangabezana nezidingo zokutholwa okusheshayo kobuthi obuningi besikhunta kusampula yobisi. Ama-detector ahlanganisiwe e-UPLC asetshenziswa kancane kancane, ngamamojula okucindezela aphezulu kanye namakholomu amancane e-chromatography yosayizi wezinhlayiya, anganciphisa isikhathi sokusebenza kwesampula, athuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi afinyelele ukuzwela okuphezulu.

I-Beijing Kwinbon inikeza izixazululo eziningi zokutholwa kobisi. Ikholomu ye-Kwinbon ye-mycotoxin immunoaffinity inokucaciswa okuphezulu, ingakwazi ukuhlonza ngokunembile izinto eziqondiwe, futhi inokuqina okuqinile nge-RSD<5%. Umthamo wayo wekholomu kanye nezinga lokuthola kabusha nakho kusezingeni eliphezulu embonini.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Aflatoxin M1 angakwazi ukubamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha nangokubanzi endaweni yokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.