Amakholomu e-China Immunoaffinity wefekthri yokuthola i-Aflatoxin M1 nabakhiqizi

Amakholomu e-Immunoaffinity okuthola i-Aflatoxin M1 Isithombe Esifakiwe

Amakholomu e-Immunoaffinity okutholwa kwe-Aflatoxin M1

Incazelo emfushane:

Amakholomu e-Kwinbon Aflatoxin M1 asetshenziswa ngokuhlanganisa ne-HPLC, LC-MS, ikhithi yokuhlola ye-ELISA.

Kungaba ukuhlolwa kwenani i-AFM1 yobisi oluwuketshezi, iyogathi, impushana yobisi, ukudla okukhethekile okudliwayo, ukhilimu noshizi.

Thumela i-imeyili kithi Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

Imininingwane yomkhiqizo

Inombolo yekati.	KH00902Z
Izakhiwo	Okokuhlola i-Aflatoxin M1
Le yindawo ka Origin	Beijing, China
I gama le-brand	Kwinbon
Usayizi Weyunithi	Izivivinyo ezingama-25 ngebhokisi ngalinye
Uhlelo lokusebenza lwesampula	Lubisi lwe-iquid, iyogathi, impushana yobisi, ukudla okukhethekile okudliwayo, ukhilimu noshizi
Isitoreji	2-30 ℃
Impilo yeshelufu	Izinyanga ezingu-12
Ukulethwa	Izinga lokushisa legumbi

Izisetshenziswa nama-reagents adingekayo

Izisetshenziswa

Ama-reagents

Izisetshenziswa

----Homogenizer -----I-Vortex mixer

----Ibhodlela lesampula ----Isilinda sokulinganisa: 10ml, 100ml

----Iphepha lokuhlunga elisezingeni/I-Centrifuge ----Ibhalansi yokuhlaziya (i-inductance: 0.01g)

----I-pipette ethweswe iziqu: 10ml ----Umjovo: 20ml

-----I-Volumetric flask: 250ml ----I-rubber pipette bulb

-----Micropipette: 100-1000ul ----Ifaneli yengilazi 50ml

----Izihlungi ze-Microfiber (Whatman, 934-AH, Φ11cm, 1.5um indilinga)

Ama-reagents

-----Methanol (AR)

---- I-Acetic acid (AR)

----I-sodium chloride (NACL,AR)

---- Amanzi akhishiwe

Izinzuzo zomkhiqizo

Amakholomu e-Kwinbon Inmmunoaffinity asebenzisa i-chromatography ewuketshezi ukuze ahlukanise, ahlanze noma ahlaziye okuthile kwe-Aflatoxin M1. Ngokuvamile amakholomu e-Kwinbon ahlanganiswa ne-HPLC.

Ukuhlaziywa kobuningi be-HPLC kobuthi besikhunta kuyindlela yokuthola abantu abadala. Kokubili i-chromatography yesigaba saphambili nesemuva iyasebenza. I-reverse phase HPLC iyonga, kulula ukuyisebenzisa, futhi inobuthi obuncibilikisiwe. Ubuthi obuningi buyancibilika ezigabeni zamaselula ezipholile bese buhlukaniswa ngamakholomu echromatography engeyona indawo epholile, ehlangabezana nezidingo zokutholwa ngokushesha kobuthi besikhunta kusampula yobisi. Izitholi ezihlanganisiwe ze-UPLC zisetshenziswa kancane kancane, ezinamamojula okucindezela okuphezulu nosayizi omncane namakholomu e-chromatography kasayizi wezinhlayiyana, ezingafinyeza isikhathi sokusebenza kwesampula, kuthuthukise ukusebenza kahle kokuhlukaniswa kwe-chromatographic, futhi kufinyelele ukuzwela okuphezulu.

I-Beijing Kwinbon inikeza izixazululo eziningi zokutholwa kobisi. Ikholomu ye-Kwinbon ye-mycotoxin immunoaffinity inokucaciswa okuphezulu, ingakwazi ukuhlonza ngokunembile izinto eziqondiwe, futhi inokuqina okuqinile nge-RSD<5%. Umthamo wayo wekholomu kanye nezinga lokuthola kabusha nakho kusezingeni eliphezulu embonini.

Ngokucaciswa okuphezulu, amakholomu e-Kwinbon Aflatoxin M1 angakwazi ukubamba ama-molecule aqondiwe esimweni esimsulwa kakhulu. Futhi amakholomu e-Kwinbon ageleza ngokushesha, kulula ukuwasebenzisa. Manje isisetshenziswa ngokushesha futhi kabanzi emkhakheni wezokudla kanye nokusanhlamvu ekukhohliseni ama-mycotoxins.