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umkhiqizo

Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin Isithombe Esifakiwe
  • Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin A
  • Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin A

Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin A

Incazelo emfushane:

Le khithi ingasetshenziswa ekuhlaziyeni inani nekhwalithi ye-ochratoxin A ekuphakeleni.Ungumkhiqizo omusha wokutholwa kwezinsalela zomuthi ngokusekelwe kubuchwepheshe be-ELISA, obiza kuphela amaminithi angu-30 ekusebenzeni ngakunye futhi onganciphisa kakhulu amaphutha okusebenza kanye nokuqina komsebenzi.Le khithi isuselwe kubuchwepheshe bokuncintisana obungaqondile be-ELISA.Imithombo ye-microtiter ihlanganiswe ne-antigen ehlangene.I-Ochratoxin A kusampula iqhudelana ne-antigen ehlanganiswe epuleti le-microtiter ye-antibody eyengezwe .Ngemuva kokwengezwa kwe-enzyme conjugate, i-substrate ye-TMB isetshenziselwa ukukhombisa umbala.Ukungabi khona kwesampula kuhlobene kabi nensalela ye-o chratoxin A kuyo, ngemva kokuqhathanisa Nejika Elijwayelekile, eliphindaphindwa izici zokuhlanjululwa, inani le-Ochratoxin A kusampula lingabalwa.


Izikhombisi-ndlela Zokusetshenziswa

Imininingwane Yomkhiqizo

Omaka bomkhiqizo

I-Ochratoxins iyiqembu lama-mycotoxin akhiqizwa ezinye izinhlobo ze-Aspergillus (ikakhulukazi A).I-Ochratoxin A yaziwa ukuthi yenzeka ezimpahleni ezifana nokusanhlamvu, ikhofi, izithelo ezomisiwe newayini elibomvu.Ibhekwa njenge-carcinogen yomuntu futhi inesithakazelo esikhethekile njengoba inganqwabelana enyameni yezilwane.Ngakho inyama nemikhiqizo yenyama ingangcoliswa ngalobu buthi.Ukuchayeka kuma-ochratoxin ngokudla kungaba nobuthi obukhulu ezinso ezincelisayo, futhi kungase kubangele umdlavuza.

Imininingwane

1. Ikhithi Yokuhlola ye-Elisa ye-Ochratoxin A

2. Ikati.KA07301H-96 Wells

3. Izingxenye zekhithi
● Ipuleti le-Microtiter elinemithombo engu-96 embozwe nge-antigen
● Izixazululo ezijwayelekile(amabhodlela angu-6:1ml/ibhodlela)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● I-Enzyme conjugate 7ml…………………………………………………………………..………..…..ikepisi elibomvu
● Isixazululo se-antibody 10ml…………………………………………………………………………….…ikepisi eliluhlaza
● Isixazululo se-substrate A 7ml…………………………………………………………………………………ikepisi elimhlophe
● Isixazululo se-Substrate B 7ml……………………………………………………………..…………………………
● Isixazululo sokumisa 7ml …………………………………………………………………………………………………
● 20×isixazululo esigxilisiwe se-Wash 40ml………..…………………………………….…………ikepisi esobala

4. Ukuzwela, ukunemba nokunemba
Ukuzwela Kokuhlola: 0.4ppb
Umkhawulo wokutholwa
Okuphakelayo………………………………………………………………………………………….… 5ppb
Ukunemba
Okuphakelayo………………………………………………………………………….….…90±20%
Ukunemba:I-Coefficient ehlukile yekhithi ye-ELISA ingaphansi kuka-10%.

5. Cross Rate
I-Ochratoxin A……………………………………………………………………..100%

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