umkhiqizo

I-Nitrofuran iyimithi elwa namagciwane yokwenziwa ebanzi, evame ukusetshenziswa ekukhiqizeni izilwane ngenxa yezakhiwo zayo ezinhle kakhulu zokulwa namagciwane kanye ne-pharmacokinetic.
Ayephinde asetshenziswa njengabagqugquzeli bokukhula kwengulube, izinkukhu kanye nokukhiqizwa kwamanzi.Ezifundweni zesikhathi eside ngezilwane zaselabhu zabonisa ukuthi izidakamizwa zomzali kanye nama-metabolite azo akhombisa izici ze-carcinogenic kanye ne-mutagenic.Izidakamizwa ze-nitrofuran i-furaltadone, i-nitrofurantoin ne-nitrofurazone zavinjelwa ukusetshenziswa ekukhiqizeni izilwane zokudla e-EU ngo-1993, futhi ukusetshenziswa kwe-furazolidone kwakuvinjelwe ku-1995.

Imininingwane

1.Ikhithi Yokuhlola ye-Elisa ye-AOZ

2.Ikati.A008-96 Wells

Izingxenye ze-3.Kit
● Ipuleti le-Microtiter elimbozwe nge-antigen, imithombo engu-96
● Izisombululo ezijwayelekile(6 amabhodlela,1ml/ibhodlela)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
● Ukulawula okujwayelekile kwe-spiking : (1ml/ibhodlela)............................................. .............100ppb
● I-enzyme conjugate concentrate 1.5ml............................................ ..….….ikepisi elibomvu
● Isixazululo se-antibody sigxiliswe ku-0.8ml………………………………..
● I-Substrate A 7ml …………………………………………. ......................................ikepisi elimhlophe
● I-Substrate B7ml…………………………………………… .....................…..ikepisi elibomvu
● Isixazululo sokumisa 7ml……………………………………………………………ikepisi eliphuzi
● 20×isixazululo sokugeza esigxilile esingu-40ml ……………………….……ikepisi elikhanyayo
● 2×isixazululo sokukhipha okugxilisiwe 60ml……………………..…………….ikepisi eluhlaza okwesibhakabhaka
● 2-Nitrobenzaldehyde 15.1mg…………………………………………………ikepisi elimnyama

4.Ukuzwela, ukunemba nokunemba
Ukuzwela: 0.025ppb
Umkhawulo wokutholwa……………..………………………………….0.1ppb
Ukunemba:
Izicubu zezilwane (imisipha nesibindi)……………………75±15%
Uju……………………………………………………..90±20%
Iqanda……………………………………………………..…90±20%
Ubisi………………………………………………………..…90±10%
Ukunemba: I-CV yekhithi ye-ELISA ingaphansi kuka-10%.

5.Cross Rate
I-Furazolidone metabolite (AOZ)……………………………………………..100%
I-Furaltadone metabolite (AMOZ)…………………………………………<0.1%
I-Nitrofurantoin metabolite (AHD)………………………………………<0.1%
I-Nitrofurazone metabolite (SEM)…………………………………………<0.1%
I-Furazolidone………………………………………………………….…..…16.3%
Furaltadone…………………………………………………………….…<1%
I-Nitrofurantoin………………………………………………………….…<1%
I-Nitrofurazone……………………………………………………………..…<1%