umkhiqizo

Mayelana

Le khithi ingasetshenziswa ekuhlaziyeni inani nekhwalithi yensalela ye-AMOZ emikhiqizweni yasemanzini (izinhlanzi nezimfanzi), njll. Ukuhlolwa kokuhlolwa kwe-enzyme, uma kuqhathaniswa nezindlela ze-chromatographic, kubonisa izinzuzo ezinkulu mayelana nokuzwela, umkhawulo wokuthola, okokusebenza kobuchwepheshe kanye nemfuneko yesikhathi.

Le khithi yakhelwe ukuthola i-AMOZ ngokusekelwe kumgomo wokuhlolwa kwe-enzyme engaqondile yokuncintisana.Imithombo ye-microtiter imbozwe nge-capture BSA exhunywe

i-antigen.I-AMOZ kusampula iqhudelana ne-antigen embozwe epuletini le-microtiter ye-antibody engeziwe.Ngemuva kokwengezwa kwe-enzyme conjugate, i-substrate ye-chromogenic iyasetshenziswa futhi isignali ikalwa nge-spectrophotometer.Ukumuncwa kuhambisana ngokuphambene nokugxilisana kwe-AM OZ kusampula.

Izingxenye Zekhithi

·I-Microtiter plate enemithombo engama-96 embozwe nge-antigen

Izixazululo ezijwayelekile (amabhodlela ayi-6)

0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb

·Isixazululo esijwayelekile se-Spiking: (1ml/ibhodlela) …………………………………………………100ppb

·I-Enzyme conjugate 1ml…………………………………………………………..…………….ikepisi elibomvu

·Isixazululo se-antibody 7ml ……………………………………………………..…………..….ikepisi eliluhlaza

Isixazululo A 7ml……………………………………………………….…………………ikepisi elimhlophe

Isixazululo B 7ml ……………………………………………………………….………..…… ikepisi elibomvu

·stop solution 7ml ………………………………………………………….…………ikepisi eliphuzi

·20×isixazululo sokugeza esigxilile 40ml……………………………………….………

·2×isixazululo sokukhipha okugxilisiwe 50ml………………………………………….…….ikepisi eluhlaza okwesibhakabhaka

·2-Nitrobenzaldehyde 15.1mg…………………………………………………………………

Ukuzwela, ukunemba nokunemba

Ukuzwela: 0.05ppb

Umkhawulo wokutholwa

Imikhiqizo yasemanzini(izinhlanzi nezimfanzi)………….………… 0.1ppb

Ukunemba

Imikhiqizo yasemanzini(izinhlanzi nezimfanzi)…………………… 95±25%

Ukunemba:I-CV yekhithi ye-ELISA ingaphansi kwe-10%.

I-Cross Rate

I-Furaltadone metabolite(AMOZ)…………………………………….…………100%

I-Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%

I-Nitrofurantoin metabolite(AHD)………………………………….…………<0.1%

I-Nitrofurazone metabolite(SEM)……………………………………………..…<0.1%

Furaltadone………………………………………………………………….…….11.1%

I-Furazolidone………………………………………………………….…...…<0.1%

I-Nitrofurantoin……………………………………………………………….…<1%

I-Nitrofurazone………………………………………………….…………………<1%