umkhiqizo

Imithamo emikhulu ye-aflatoxin iholela ebuthini obunzima (i-aflatoxicosis) obungaba yingozi empilweni, ngokuvamile ngokulimala kwesibindi.
I-Aflatoxin B1 iyi-aflatoxin ekhiqizwa i-Aspergillus flavus kanye ne-A. parasiticus.Kuyi-carcinogen enamandla kakhulu.La mandla e-carcinogenic ayahlukahluka kuzo zonke izinhlobo zezilwane nezinye, njengamagundane nezinkawu, abonakala esengozini kakhulu kunezinye.I-Aflatoxin B1 iwukungcola okuvamile ekudleni okunhlobonhlobo okuhlanganisa amakinati, impuphu yekotoni, ummbila, nokunye okusanhlamvu;kanye nokudla kwezilwane.I-Aflatoxin B1 ithathwa njenge-aflatoxin enobuthi kakhulu futhi ibandakanyeka kakhulu ku-hepatocellular carcinoma (HCC) kubantu.[catation edingekayo] Ezilwaneni, i-aflatoxin B1 nayo iboniswe njenge-mutagenic, teratogenic, futhi ibangela ukucindezelwa kwamasosha omzimba.Izindlela ezimbalwa zokusampula nezindlela zokuhlaziya ezihlanganisa i-thince-layer chromatography (TLC), i-high-performance liquid chromatography (HPLC), i-mass spectrometry, kanye ne-enzyme-linked immunosorbent assay (ELISA), phakathi kokunye, isetshenziselwe ukuhlola ukungcoliswa kwe-aflatoxin B1 ekudleni. .Ngokusho kweNhlangano Yezokudla Nezolimo (i-FAO), amazinga aphezulu okubekezelelwa emhlabeni wonke e-aflatoxin B1 kubikwe ukuthi aphakathi kuka-1-20 μg/kg ekudleni, kanye no-5-50 μg/kg ekudleni kwezinkomo ngo-2003.

Imininingwane

1.I-Elisa Test Kit ye-Aflatoxin B1

2.Ikati.I-KA07202H-96wells

3. Izingxenye zekhithi
● I-Microtiter plateprecoated ne-antigen, imithombo engu-96
● Isixazululo Esijwayelekile ×6ibhodlela(1ml/ibhodlela)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● I-Enzyme conjugate 7ml……………………………………………………………..…………ikepisi elibomvu
● Isixazululo se-antibody7ml............................................... ................................................................
● I-Substrate A 7ml……………………………………………………………………………………ikepisi elimhlophe
● I-Substrate B 7ml……………….………………………………………………………………………
● Isixazululo sokumisa 7ml……….…….…………………………………………………..…………ikepisi eliphuzi
● 20×isixazululo sokugeza esigxilisiwe esingu-40ml ………………………………………………ikepisi esobala
● 2×isixazululo sokukhipha okugxilisiwe 50ml……………………………………………………ikepisi eliluhlaza okwesibhakabhaka

4.Ukuzwela, ukunemba nokunemba
Ukuzwela: 0.05ppb

5.Umkhawulo wokutholwa
Isampula yamafutha adliwayo .......................................... .................................................. .......................0.1ppb
I-Peanut................................................. .................................................. .......................0.2ppb
Okusanhlamvu................................................. .................................................. .....................0.05ppb
Ukunemba
Isampula yamafutha adliwayo .......................................... .................................................. .................80±15%
I-Peanut................................................. .................................................. ....................80±15%
Okusanhlamvu................................................. .................................................. ....................80±15%
Ukunemba:I-Coefficient ehlukile yekhithi ye-ELISA ingaphansi kuka-10%.

6.Cross Rate
I-Aflatoxin B1 ··················100%
I-Aflatoxin B2 ·······················81 .3%
I-Aflatoxin G1 ·······················62%
I-Aflatoxin G2 ·······················22.3%