Apo Idanwo ELisa ti AOZ
Nipa
Ohun elo yii le ṣee lo ni pipo ati itupalẹ agbara ti iyoku AOZ ninu awọn ẹran ara ẹran (adie, ẹran, ẹlẹdẹ, bbl), wara, oyin ati awọn eyin.
Onínọmbà ti aloku awọn oogun nitrofuran nilo lati da lori wiwa ti awọn metabolites ti ara ti awọn oogun obi nitrofuran, eyiti o pẹlu Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) ati Nitrofurazone metabolite (SEM).
Ti a ṣe afiwe pẹlu awọn ọna chromatographic, ohun elo wa ṣafihan awọn anfani pupọ nipa ifamọ, opin wiwa, ohun elo imọ-ẹrọ ati ibeere akoko.
Ohun elo Kit
• Microtiter awo ti a bo pẹlu antijeni, 96 kanga
• Awọn ojutu boṣewa (igo 6, 1 milimita / igo)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Spiking boṣewa Iṣakoso: (1ml/igo).....................................................….100ppb
• Enzyme conjugate idojukọ 1.5ml ....................................................…….fila pupa
Ojutu Antibody ni ogidi 0.8ml………………………………………………...... fila alawọ ewe
• Sobusitireti A 7ml.................................................................................. fila funfun
• Sobusitireti B7ml………………………………………………................................................ fila pupa
Ojutu iduro 7ml……………………………………………………………….……… fila ofeefee
• 20× ogidi w ojutu 40ml ………………………………………. fila sihin
• Ojutu isediwon 2× ogidi 60ml………………………..………………. fila buluu
2-Nitrobenzaldehyde 15.1mg………………………………………………………………
Ifamọ, išedede ati konge
Ifamọ: 0.025ppb
Iwọn wiwa…………………………………………………………………… 0.1ppb
Yiye:
Ẹran ara (isan ati ẹdọ) ………………………………… 75± 15%
Oyin……………………………………………………………………………………………………………….90±20%
Ẹyin ....................
Wara ............................................................. .. ...
Itọkasi:CV ti ohun elo ELISA ko kere ju 10%.
Agbekọja Oṣuwọn
Furazolidone metabolite (AOZ) …………………………………………………………………………………………..
Furaltadone metabolite (AMOZ) …………………………………………………………………
Nitrofurantoin metabolite (AHD)………………………………………………………
Nitrofurazone metabolite (SEM) …………………………………………………………………………………………….. <0.1%
Furazolidone ……………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
Furaltadone………………………………………………………………………………………………………………………………………………………
Nitrofurantoin……………………………………………………………………………………….
Nitrofurazone…………………………………………………………………………………………………………………………………………………