Apo Idanwo Elisa ti Aflatoxin B1
Awọn iwọn nla ti aflatoxins yori si majele nla (aflatoxicosis) ti o le jẹ idẹruba igbesi aye, nigbagbogbo nipasẹ ibajẹ si ẹdọ.
Aflatoxin B1 jẹ aflatoxin ti a ṣe nipasẹ Aspergillus flavus ati A. parasiticus.O jẹ carcinogen ti o lagbara pupọ.Agbara carcinogenic yii yatọ si awọn eya pẹlu diẹ ninu, gẹgẹbi awọn eku ati awọn obo, ti o dabi ẹnipe o ni ifaragba ju awọn miiran lọ.Aflatoxin B1 jẹ idoti ti o wọpọ ni ọpọlọpọ awọn ounjẹ pẹlu ẹpa, ounjẹ owu, agbado, ati awọn irugbin miiran;bakannaa awọn ifunni ẹran.Aflatoxin B1 ni a gba aflatoxin ti o majele julọ ati pe o ni ipa pupọ ninu carcinoma hepatocellular (HCC) ninu eniyan.Ọpọlọpọ awọn iṣapẹẹrẹ ati awọn ọna itupalẹ pẹlu chromatography tinrin-Layer (TLC), chromatography olomi-giga (HPLC), spectrometry pupọ, ati imunosorbent immunosorbent ti sopọ mọ enzymu (ELISA), laarin awọn miiran, ni a ti lo lati ṣe idanwo fun aflatoxin B1 kontaminesonu ninu awọn ounjẹ. .Gẹgẹbi Ajo Ounje ati Iṣẹ-ogbin (FAO), awọn ipele ifarada ti o pọju agbaye ti aflatoxin B1 ni a royin pe o wa ni iwọn 1–20 μg/kg ninu ounjẹ, ati 5–50 μg/kg ninu ifunni ẹran-ọsin ni ọdun 2003.
Awọn alaye
1.Elisa Apo Idanwo fun Aflatoxin B1
2.Ológbò.KA07202H-96kanga
3. Kit irinše
● Microtiter platecoated pẹlu antijeni, 96 kanga
● Solusan Standard ×6 igo (1 milimita/igo)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml…………………………………………………………………………………………. fila pupa
● Ojutu antibody7ml............................................ ...................................... fila alawọ ewe
● Sobusitireti A 7ml……………………………………………………………………………………………………… fila funfun
● Sobusitireti B 7ml…………………………………………………………………………………………………. fila pupa
● Idaduro ojutu 7ml……………………………………………………………………………………………………………… fila ofeefee
● 20× ogidi fifọ ojutu 40ml ………………………………………………………… fila sihin
● 2× ojutu isediwon ogidi 50ml……………………………………………………………………… fila bulu
4.Sensitivity, išedede ati konge
Ifamọ: 0.05ppb
5.Detection ifilelẹ
Apeere epo toje................................................. ................................................. .........................0.1ppb
Epa................................................. ................................................. ......................0.2ppb
Irugbin................................................. ................................................. ...........0.05ppb
Yiye
Apeere epo toje................................................. ................................................. ...................80± 15%
Epa................................................. ................................................. ......................80± 15%
Irugbin................................................. ................................................. ......................80± 15%
Itọkasi:Olusọdipúpọ iyatọ ti ohun elo ELISA ko kere ju 10%.
6.Cross Rate
Aflatoxin B1·······················100%
Aflatoxin B2········································81 .3%
Aflatoxin G1···························62%
Aflatoxin G2·········································22.3%