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Apo Idanwo Elisa ti Aflatoxin B1 Ti Afihan Aworan
  • Apo Idanwo Elisa ti Aflatoxin B1
  • Apo Idanwo Elisa ti Aflatoxin B1
  • Apo Idanwo Elisa ti Aflatoxin B1

Apo Idanwo Elisa ti Aflatoxin B1

Apejuwe kukuru:

Awọn iwọn nla ti aflatoxins yori si majele nla (aflatoxicosis) ti o le jẹ idẹruba igbesi aye, nigbagbogbo nipasẹ ibajẹ si ẹdọ.

Aflatoxin B1 jẹ aflatoxin ti a ṣe nipasẹ Aspergillus flavus ati A. parasiticus.O jẹ carcinogen ti o lagbara pupọ.Agbara carcinogenic yii yatọ si awọn eya pẹlu diẹ ninu, gẹgẹbi awọn eku ati awọn obo, ti o dabi ẹnipe o ni ifaragba ju awọn miiran lọ.Aflatoxin B1 jẹ idoti ti o wọpọ ni ọpọlọpọ awọn ounjẹ pẹlu ẹpa, ounjẹ owu, agbado, ati awọn irugbin miiran;bakannaa awọn ifunni ẹran.Aflatoxin B1 ni a gba aflatoxin ti o majele julọ ati pe o ni ipa pupọ ninu carcinoma hepatocellular (HCC) ninu eniyan.Ọpọlọpọ awọn iṣapẹẹrẹ ati awọn ọna itupalẹ pẹlu chromatography tinrin-Layer (TLC), chromatography olomi-giga (HPLC), spectrometry pupọ, ati imunosorbent immunosorbent ti sopọ mọ enzymu (ELISA), laarin awọn miiran, ni a ti lo lati ṣe idanwo fun aflatoxin B1 kontaminesonu ninu awọn ounjẹ. .Gẹgẹbi Ajo Ounje ati Iṣẹ-ogbin (FAO), awọn ipele ifarada ti o pọju agbaye ti aflatoxin B1 ni a royin pe o wa ni iwọn 1–20 μg/kg ninu ounjẹ, ati 5–50 μg/kg ninu ifunni ẹran-ọsin ni ọdun 2003.


Awọn Itọsọna Fun Lilo

Alaye ọja

ọja Tags

Nipa

Ohun elo yii le ṣee lo fun agbara ati itupalẹ pipo ti aflatoxin B1 ninu epo ti o jẹun, ẹpa, awọn oka cereal, obe soy, kikan ati ifunni (kikọ sii aise, awọn ohun elo ipele ti o dapọ ati awọn ohun elo ogidi). itupale ohun elo.

Ọja yii da lori ELISA ifigagbaga aiṣe-taara, eyiti o yara, deede ati ifarabalẹ ni akawe pẹlu itupalẹ ohun elo aṣa.O nilo iṣẹju 45 nikan ni iṣẹ kan, eyiti o le dinku aṣiṣe iṣẹ ni riro ati kikankikan iṣẹ.

Awọn paati ohun elo

• Microtiter platecoated pẹlu antijeni, 96 kanga

• Solusan Standard ×6 bottle(1ml/igo)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

Enzyme conjugate 7ml…………………………………………………………………………………………. fila pupa

• Ojutu antibody7ml............................................................ .................... fila alawọ ewe

Sobusitireti A 7ml……………………………………………………………………………….…………………. fila funfun

Sobusitireti B 7ml……………………………………………………………………………………….………… fila pupa

Idaduro ojutu 7ml……………………………………………………………………………………………….......... fila ofeefee

• 20× ogidi w ojutu 40ml …………………………………………………………. fila sihin

• 2× ojutu isediwon ogidi 50ml………………………………………………………………………………………… fila bulu

Ifamọ, išedede ati konge

Ifamọ:0.05ppb

Iwọn wiwa

Apeere epo toje................................................. .................................................................................0.1ppb

Epa................................................. ................................................. ......................0.2ppb

Irugbin................................................. ................................................. ...........0.05ppb

Yiye

Apeere epo toje................................................. .............................................................................80± 15%

Epa................................................. ................................................. ......................80± 15%

Irugbin................................................. ................................................. ......................80± 15%

Itọkasi

Olusọdipúpọ iyatọ ti ohun elo ELISA ko kere ju 10%.

Agbekọja Oṣuwọn

Aflatoxin B1····························100%

Aflatoxin B2·········································81 .3%

Aflatoxin G1···························62%

Aflatoxin G2···········································22.3%

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