imveliso

Inkcazelo emfutshane:

Le khithi ye-ELISA yenzelwe ukufumanisa i-quinolones ngokusekelwe kumgaqo-nkqubo we-enzyme immunoassay engathanga ngqo. Imithombo ye-microtiter ifakwe kwi-antigen edibeneyo ye-BSA. I-Quinolones kwisampulu ikhuphisana ne-antigen eqatywe kwipleyiti ye-microtitre ye-antibody. Emva kokongezwa kwe-enzyme conjugate, i-substrate ye-chromogenic isetyenziswa kwaye umqondiso ulinganiswa nge-spectrophotometer. Ukufunxa kuhambelana ngokuphambeneyo noxinaniso lwe-quinolones kwisampulu.