Iindaba

Ndisemva kokukhula kakhulu kwemicimbi yokhuseleko lokutya, uhlobo olutsha lwe-kitikhi ye-kiti esekwe kwiI-Enzyme-i-Insoncesbent Assay (Elisa)Ngokuthe ngcembe ube sisixhobo esibalulekileyo kwicandelo loKhuseleko loKutya. Ayiboneleli kuphela iindlela ezichanekileyo kwaye zisebenza ngokufanelekileyo kuvavanyo olusemgangathweni wokutya kodwa nekwakhela umgca wokhuselo owodwa wokukhuseleka kokutya kwabathengi.

Umgaqo we-ELISA test kit ibubuxoki ngokusebenzisa i-Antign kunye ne-anticture ukuba ichonge imixholo yezinto ekujoliswe kuzo ekutyeni kombala we-enzyme. Inkqubo yayo yokusebenza ilula kwaye inobuqhetseba okuphezulu kunye nemvakalelo, ivumelana ngokuchanekileyo kunye nemilinganiselo yezinto eziyingozi ekutyeni, ezinje nge-Aflatoxin, i-Ochratoxin A, kwayeI-T-2 toxins.

Ngokweenkqubo ezithile zokusebenza, iKita Vita ye-ELISA ngokwesiqhelo ibandakanya la manyathelo alandelayo:

I-1. Okokuqala, isampulu yokutya ukuze kuvavanywe kufuneka kulungiswe ngokufanelekileyo, njengokukhutshwa kunye nokucocwa, ukufumana isisombululo sesampulu esinokusetyenziselwa ukufumanisa.

2. Isampulu yongezelelo: Isisombululo sesampulu esiqinisekisiweyo songezwa kumaphondo otyunjiweyo kwiplate ye-ESALISA, nganye nganye ihambelana nento ekufuneka ihlolwe.

I-3. I-Incubation: IPlata ye-ELISA eyongezelelweyo ifakwe kwiqondo lobushushu eliqinisekileyo kangangexesha elithile lokuvumela ukubopha okugcweleyo phakathi kwee-antibns kunye nee-antibodies.

I-4. Ukuhlamba: Emva kokufakwa, isisombululo sokuhlamba sisetyenziselwa ukususa i-agogi ye-agoner okanye i-antibodies, ukunciphisa ukuphazamiseka kwe-nonpecific.

5.Umdibaniso wongezwa kunye nophuhliso lwemibala: Isisombululo se-subsere songezwa iqula ngalinye, kwaye i-enzyme kwi-enzyme-ilebheli efakwe i-antidmed ithathe i-antids

I-6. Ukulinganisa: Ixabiso le-absorbhation lemveliso enemibala kwiqumrhu ngalinye lilinganiswa ngokusebenzisa izixhobo ezinje ngomfundi we-ELISA. Umxholo wento ekufuneka uvavanyiwe emva koko ubalwe ngokusekwe kwijika eliqhelekileyo.