China Immunoaffinity columns for Zearalenone ubhaqo umzi-mveliso kunye nabavelisi

Imihlathi ye-Immunoaffinity ye-Zearalenone ubhaqo oFakelweyo Umfanekiso

Iintsika ze-Immunoaffinity zokufumanisa i-Zearalenone

Inkcazelo emfutshane:

Iikholamu zeKwinbon Zearalenone zisetyenziswa ngokudibanisa neHPLC, LC-MS, ELISA test kit. Ingaluvavanyo lobungakanani be-AFB1 yeenkozo kunye neemveliso eziziinkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, iimbotyi zesoya, iimbewu ezidlwengulwayo kunye neoli yemifuno, ukondla izixhobo ezikrwada kunye neemveliso ezigqityiweyo, njl.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH00304Z
Iipropati	KubaZearalenoneuvavanyo
Indawo yeMvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Iinkozo kunye neemveliso eziziinkozo, i-soy sauce, iviniga, iimveliso zesosi, utywala, iimbotyi zesoya, i-rapeseed kunye ne-oyile yemifuno, ukondla izinto ezikrwada kunye neemveliso ezigqityiweyo, njl.
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

I-Zearalenone (ZEN), eyaziwa ngokuba yi-RAL kunye ne-F-2 mycotoxin, yimetabolite enamandla ye-estrogenic eveliswayo. Iyakwazi ukumelana nobushushu yaye ifumaneka ehlabathini lonke kwizityalo eziziinkozo ezininzi, ezinjengombona, irhasi, ihabile, ingqolowa, irayisi namazimba.

I-Zearalenone yityhefu ephambili ebophelela kwi-estrogen receptors, ebangela ubudlolo, ukukhupha isisu okanye ezinye iingxaki zokuzala, ngakumbi kwiihagu.

I-Wikipedia icebisa ezi ndlela zilandelayo zokubona;

Umaleko omncinci wechromatography,
Uvavanyo lwe-immunosorbent olunxulumene ne-enzyme
I-Immunoaffinity column fluorescence
Ikholamu ye-Immunoaffinity yokusebenza okuphezulu kwechromatography yolwelo

Kwinbon Inmmunoaffinity Columns yeyesithathu indlela, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lweZearalenone. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngokucacileyo okuphezulu, iikholamu zeKwinbon Zearalenone zinokubamba iimolekyuli ekujoliswe kuzo kwindawo ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.