China Iikholamu Immunoaffinity for Vomitoxin ubhaqo umzi-mveliso kunye nabavelisi

Iintsika ze-Immunoaffinity zokubhaqa iVomitoxin Umfanekiso ofakiweyo

Iikholamu ze-Immunoaffinity zokufumanisa iVomitoxin

Inkcazelo emfutshane:

Iikholamu zeKwinbon Vomitoxin zisetyenziswa ngokudibanisa neHPLC, LC-MS, ELISA test kit.Ingaluvavanyo lobungakanani be-AFB1 yeesiriyeli, amandongomane kunye neemveliso zawo, ioyile yemifuno kunye namafutha, iimveliso zendongomane, isosi yesoya, iviniga, amayeza aseTshayina, izinongo kunye neti.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH00203Z
Iipropati	Kuvavanyo lweVomitoxin
Indawo yeMvelaphi	eBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Iisiriyeli, amandongomane kunye neemveliso zawo, ioyile yemifuno kunye namafutha, iimveliso zenut, isosi yesoya, iviniga, amayeza aseTshayina, iziqholo kunye neti.
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

IVomitoxin, ekwaziwa ngokuba yideoxynivalenol (DON), yi epoxy-sesquiterpenoid. Yenzeka kakhulu kwiinkozo ezinje ngengqolowa, irhasi, i-oats, irye, nombona, kwaye kaninzi erayisini, amazimba, kunye ne-triticale, ebangela ukugabha kunye ne-anorexia kwizilwanyana kwaye kunokukhokelela kwisicaphucaphu kunye nokugabha ebantwini.

I-Wikipedia icebisa ezi ndlela zilandelayo zokubona;

Umaleko omncinci wechromatography,
Uvavanyo lwe-immunosorbent olunxulumene ne-enzyme
I-Immunoaffinity column fluorescence
Ikholamu ye-Immunoaffinity yokusebenza okuphezulu kwechromatography yolwelo

Kwinbon Inmmunoaffinity Columns yeyesithathu indlela, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lweVomitoxin. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngokucacileyo okuphezulu, iikholamu zeKwinbon Vomitoxin zinokubamba iimolekyuli ekujoliswe kuzo kwindawo ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.