Iintsika ze-China Immunoaffinity ze-T-2 zokubona umzi-mveliso we-mycotoxin kunye nabavelisi

Imihlathi ye-Immunoaffinity ye-T-2 ubhaqo lwe-mycotoxin Umfanekiso ofakiweyo

Iikholamu ze-Immunoaffinity zokufumanisa i-T-2 mycotoxin

Inkcazelo emfutshane:

Iikholamu ze-Kwinbon T-2 ze-mycotoxin zisetyenziswa ngokudibanisa ne-HPLC, LC-MS, ELISA test kit.Inokuba luvavanyo lobungakanani be-T-2 mycotoxin yeenkozo kunye neemveliso eziziinkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, iimbotyi zesoya, ii-oyile ezidlwengulwayo kunye nemifuno, ukondla imathiriyeli ekrwada kunye neemveliso ezigqityiweyo, njl.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH01301Z
Iipropati	KubaT-2 mycotoxinuvavanyo
Indawo yeMvelaphi	EBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Iinkozo kunye neemveliso eziziinkozo, i-soy sauce, iviniga, iimveliso zesosi, utywala, njl.
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

I-T-2 i-mycotoxin sisiphumo sendalo sokungunda seFusarium spp. umngundo kwaye iyityhefu ebantwini nakwezinye izilwanyana. Imeko yeklinikhi eyibangelayo yi-alimentary toxic aleukia kunye nentaphane yeempawu ezinxulumene namalungu ahlukeneyo njengolusu, umoya kunye nesisu. Ikho kwi-T-2 mycotoxins ezingcolisekileyo iinkozo, ukutya kunye nokutya.

Kwinbon Inmmunoaffinity Columns yeyendlela yesithathu, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lwe-T-2 mycotoxin. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngokuchaneka okuphezulu, iikholamu zeKwinbon T-2 mycotoxin zinokubamba iimolekyuli ekujoliswe kuzo kwimeko esulungekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.