I-China i-tunoafinity colomus colomus ye-ochratoxin yomzi-mveliso wokuchonga kunye nabavelisi

Ubumsungululi beekholamu ze-ochratoxin of fictions umfanekiso

Ubumsulungeko lweekholamu ze-ochratoxin off

Inkcazo emfutshane:

I-Ochratoxin yekholamu isetyenziswa ngokudibanisa ne-HPPC, lc-Nks, ikhithi yovavanyo lwe-ELISA. Inokuba luvavanyo lobungakanani i-ochratoxin a ngenxa yeemveliso zengqolowa, i-gerace yesosi, i-soyso, iviniga, i-SOCUCE, i-SOCUCE, i-SOCUCE, COCOA kunye nekofu, njl.

Thumela i-imeyile kuthi Imikhombandlela yokusetyenziswa

Iinkcukacha zemveliso

Iimpawu zeMveliso

Imveliso yemveliso

Ikati no.	Kh00404z
Iipropathi	KubaI-Ochratoxin A Ukuvavanywa
Indawo yemvelaphi	IBeijing, China
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 ngebhokisi nganye
Isicelo seSampula	GIimvula kunye neemveliso zokudla okuziinkozo, isosi yengqolowa, i-Soy Sauce, iviniga, iimveliso zeSauce, i-Cocoa kunye neKofu, njl.
Ukugcinwa	2-30 ℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Igumbi le-temepe

Izixhobo kunye neRegednts eziyimfuneko

Izixhobo

Iirejista

Izixhobo

---- I-Homogenizer ---- Umxube we-PRTEX

---- Ibhotile yebhotile ---- isilinda sokulinganisa: 10ml, 100ml

---- Iphepha lokucoca ifiloti / i-Centrifuge --- ibhalansi ye-ANCHECT

---- I-Pipette ephumeleleyo: 10ml ---- i-Inactor: 20ml

---- I-dayilic flask: 250ml ---- i-raberte pipebette

---- i-micropipette: 100-1000ul ---- i-funnel ye-50ml

---- Iifilitha ze-microfiber (i-teman, i-934-Ah, φ11cm, 1.5um Isangqa)

Iirejista

---- i-metanol (i-AR)

---- i-acetic acid (i-AR)

---- I-sodium chloride (i-NACL, AR)

---- amanzi

Izibonelelo zeMveliso

Njengoko eyaziwa i-mycotoxin, ochratoxin a (o ye-OTA) eveliswa ziintlobo ezahlukeneyo zefunga ezibandakanya i-Aspergillis Ochraceus, A. Carbondarius, A. Niger kunye nePenicillium Verrucosum. I-OTA ibangela iNephrototity kunye ne-renter sumumpers kwiintlobo ngeentlobo zezilwanyana; Nangona kunjalo, iziphumo zempilo zabantu azibonakali kakuhle.

I-Kwinbon Inmmmophafmoafinity yendlela yendlela yesithathu, isebenzise i-chromatography ye-chromatography, ukucocwa okanye uhlalutyo oluthile lwe-Ochratoxin A.

Uhlalutyo lobungakanani be-HPPL ye-fungal toxins yindlela yokufumana iqonga. Zombini ezihamba phambili kunye nokubuyela umva kwi-chromatography yeSigaba isebenza. I-HLLC yeSigaba se-HPLC inoqoqosho, kulula ukusebenza, kwaye inobuthathaka obuphantsi. Uninzi lweetyhefu zinyibilikisi kwizigaba ze-polars ze-Colar kwaye zahlulwe yikholamu ye-chromatography, hlanganisa iimfuno zokufunyanwa ngokukhawuleza kweetyhubhu ezininzi zethayile. I-UPLC edibeneyo ngokuthe ngcembe isetyenziswa ngokuthe ngcembe, kunye neemodyuli zoxinzelelo oluphezulu kunye nobukhulu obuncinci kwaye zinokuphelisa isampulu yexesha elifanelekileyo, kwaye liphumelele isampulu yokwahlulahlula, kwaye iphumelele imvakalelo ephezulu.

Ngobuchule obuphezulu, kwi-winbon ochratoxin Iikholamu zinokubambisa iimolekyuli ezijolise kwimeko ecocekileyo. Iikholan zeKinbon ziyahamba ngokukhawuleza, i-EASSY ukuba isebenze. Ngoku kukhawuleze kwaye usebenzisa kakhulu kwi-sull kunye nentsimi yengqolowa yenkohliso ye-Mycotoxins.