China Iinmunoaffinity columns for Ochratoxin Umzi-mveliso wobhaqo kunye nabavelisi

Iintsika ze-Immunoaffinity ze-Ochratoxin A ukufunyaniswa koMfanekiso obonakalayo

Iikholamu ze-Immunoaffinity zokufumanisa i-Ochratoxin A

Inkcazelo emfutshane:

Iikholamu zeKwinbon Ochratoxin A zisetyenziswa ngokudibanisa neHPLC, LC-MS, ELISA test kit. Ingaluvavanyo lobungakanani be-Ochratoxin A yeenkozo kunye neemveliso eziziinkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, iCocoa kunye nekofu egcadiweyo, njl.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH00404Z
Iipropati	KubaOchratoxin A uvavanyo
Indawo yeMvelaphi	eBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Giimvula kunye neemveliso eziziinkozo, isosi yesoya, iviniga, iimveliso zesosi, utywala, ikokho kunye nekofu egcadiweyo, njl.
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

Njengoko yaziwa ngokuba yi-mycotoxin, i-Ochratoxin A (OTA) eveliswe zintlobo ezininzi zokungunda eziquka i-Aspergillus ochraceus, A. carbonarius, A. niger kunye nePenicillium verrucosum. I-OTA ibangela i-nephrotoxicity kunye namathumba ezintso kwiintlobo ngeentlobo zezilwanyana; nangona kunjalo, iziphumo zempilo yabantu azibonakali kakuhle.

Kwinbon Inmmunoaffinity Columns yeyesithathu indlela, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lwe-Ochratoxin A. Ngokwesiqhelo iikholamu zeKwinbon zidityaniswa ne-HPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngokuchaneka okuphezulu, iikholamu zeKwinbon Ochratoxin A zinokubamba iimolekyuli ekujoliswe kuzo kwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.