China Immunoaffinity columns for Fumonisin ubhaqo umzi-mveliso kunye nabavelisi

Imihlathi ye-Immunoaffinity yokufunyanwa kweFumonisin Umfanekiso ofakiweyo

Iintsika ze-Immunoaffinity zokufumana i-Fumonisin

Inkcazelo emfutshane:

Iikholamu zeKwinbon Fumonisin zisetyenziswa ngokudibanisa neHPLC, LC-MS, ELISA test kit. Ilungele ukutya okuziinkozo, ukutya, amayeza aseTshayina, njl njl kwaye iphucula ukucoceka kweesampulu.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH01001Z
Iipropati	KubaFumonisinuvavanyo
Indawo yeMvelaphi	eBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Ukutya, iiCereal, iiNkozo kunye neziqholo
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

I-Fomonisin(FB) luhlobo lweemetabolites ezinyibilikayo emanzini eziveliswa kwi-toxigenic fungi. Ingcolisa ubukhulu becala iinkozo kunye neemveliso zazo, kwaye inetyhefu eyingozi kakhulu kunye ne-carcinogenicity enokubakho kwimfuyo ethile, kwaye ibe yenye indawo yophando emva kwe-aflatoxin. Inokubangela umonakalo omkhulu ebantwini nakwizilwanyana ukuba le nto ibonakaliswe ngokubanzi kumbona nakwezinye iimveliso zezolimo.Kutshanje i-fumonisin iye yaba yenye yezona zinto zingcolisekileyo zehlabathi. ihlabathi.

I-Wikipedia icebisa ezi ndlela zilandelayo zokubona;

Umaleko omncinci wechromatography,
Uvavanyo lwe-immunosorbent olunxulumene ne-enzyme
I-Immunoaffinity column fluorescence
Ikholamu ye-Immunoaffinity yokusebenza okuphezulu kwechromatography yolwelo

Kwinbon Inmmunoaffinity Columns yeyesithathu indlela, isebenzisa ulwelo lwechromatography ukwahlula, ukucocwa okanye uhlalutyo oluthile lweFumonisin. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.

Uhlalutyo lobungakanani be-HPLC yetyhefu yokungunda bubuchule bokubona obuqolileyo. Zombini i-chromatography yesigaba sangaphambili kunye nesigaba esingasemva siyasebenza. Isigaba sokubuyela umva i-HPLC inoqoqosho, kulula ukuyisebenzisa, kwaye inetyhefu ye-solvent ephantsi. Uninzi lwee-toxins ziyanyibilika kwizigaba ezihamba ngepolar kwaye emva koko zahlulwe ziikholamu zechromatography ezingeyopolar, zihlangabezana neemfuno zokubhaqwa ngokukhawuleza kweetoxin ezininzi zefungal kwisampulu yobisi. I-UPLC i-detectors edibeneyo isetyenziswa ngokuthe ngcembe, kunye neemodyuli eziphezulu zoxinzelelo kunye nobukhulu obuncinci kunye neekholamu ze-chromatography ze-particle size inokunciphisa ixesha lokuhamba kwesampuli, ukuphucula ukusebenza kakuhle kokwahlula kwechromatographic, kunye nokufezekisa uvakalelo oluphezulu.

Ngokuchaneka okuphezulu, iikholamu zeKwinbon Fumonisin zinokubamba iimolekyuli ekujoliswe kuzo kwindawo ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.