I-China i-tunoafinity colomns yomzi-mveliso wokuchonga i-fundonisin kunye nabavelisi

Ikholamu -ffinity colomus yeFumonisin yokufumana umfanekiso

Ikholamu -ffinity colomus ye-Fuminisin yokufunyanwa

Inkcazo emfutshane:

I-Kwinbon Fimonisosin Iikholamu zisetyenziswa ngokudibanisa ne-HPPC, lc-Nks, ikhithi yovavanyo lwe-Elisa. Ilungele ukhula, ukutya, amayeza aseTshayina, njl njl kwaye kuphucula ubunyulu beesampulu.

Thumela i-imeyile kuthi Imikhombandlela yokusetyenziswa

Iinkcukacha zemveliso

Iimpawu zeMveliso

Imveliso yemveliso

Ikati no.	Kh01001z
Iipropathi	KubaI-fumonisinUkuvavanywa
Indawo yemvelaphi	IBeijing, China
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 ngebhokisi nganye
Isicelo seSampula	Ukutya, ukutya okuziinkozo, ukutya okuziinkozo kunye neziqholo
Ukugcinwa	2-30 ℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Igumbi le-temepe

Izixhobo kunye neRegednts eziyimfuneko

Izixhobo

Iirejista

Izixhobo

---- I-Homogenizer ---- Umxube we-PRTEX

---- Ibhotile yebhotile ---- isilinda sokulinganisa: 10ml, 100ml

---- Iphepha lokucoca ifiloti / i-Centrifuge --- ibhalansi ye-ANCHECT

---- I-Pipette ephumeleleyo: 10ml ---- i-Inactor: 20ml

---- I-dayilic flask: 250ml ---- i-raberte pipebette

---- i-micropipette: 100-1000ul ---- i-funnel ye-50ml

---- Iifilitha ze-microfiber (i-teman, i-934-Ah, φ11cm, 1.5um Isangqa)

Iirejista

---- i-metanol (i-AR)

---- i-acetic acid (i-AR)

---- I-sodium chloride (i-NACL, AR)

---- amanzi

Izibonelelo zeMveliso

Ifonisistin (FB) luhlobo lwee-metaboltes zamanzi ezinyibilikisiweyo ezivela kwi-toxigenic fungi. Ichasene nobudenge kunye neemveliso zabo, kwaye unetyhefu yetyhefu ye-carcinogeniciction kunye ne-carcinogenicicgenicicgenity kwimfuyo ethile, kwaye iye yaba lolunye uhlobo lophando emva kwe-Aflatoxin. Inokubangela ukonakala okukhulu ebantwini kunye nezilwanyana eziboniswe ngokubanzi kwingqolowa kunye nezinye iimveliso zezolimo. Umhlaba.

I-Wikipedia icebisa ngeendlela ezichongiweyo;

I-thermar chromatography,
I-Enzyme-i-Insoncerbent Assay
Ikholamu yeKholamu yeKholamu ye-Fluorescence
Ikholamu yeKholamu yokuSebenzisa i-CHROMATOPY YOLWILE

I-Kwinbon Inmmnofinity ikhomishini yesithathu yendlela, isebenzise i-chromatography ye-chromatography yokwahlulahlula, ukucocwa okanye uhlalutyo oluthile lwe-fumuminisin. Ngokwesiqhelo iikholamu zeKinbon zidityaniswa ne-HPPC.

Uhlalutyo lobungakanani be-HPPL ye-fungal toxins yindlela yokufumana iqonga. Zombini ezihamba phambili kunye nokubuyela umva kwi-chromatography yeSigaba isebenza. I-HLLC yeSigaba se-HPLC inoqoqosho, kulula ukusebenza, kwaye inobuthathaka obuphantsi. Uninzi lweetyhefu zinyibilikisi kwizigaba ze-polars ze-Colar kwaye zahlulwe yikholamu ye-chromatography, hlanganisa iimfuno zokufunyanwa ngokukhawuleza kweetyhubhu ezininzi zethayile. I-UPLC edibeneyo ngokuthe ngcembe isetyenziswa ngokuthe ngcembe, kunye neemodyuli zoxinzelelo oluphezulu kunye nobukhulu obuncinci kwaye zinokuphelisa isampulu yexesha elifanelekileyo, kwaye liphumelele isampulu yokwahlulahlula, kwaye iphumelele imvakalelo ephezulu.

Ngobunye obuphezulu, kwi-Kwinbon fumonisin Iikholamu zinokubambisa iimolekyuli ezijolise kwimeko ecocekileyo. Iikholan zeKinbon ziyahamba ngokukhawuleza, i-EASSY ukuba isebenze. Ngoku kukhawuleze kwaye usebenzisa kakhulu kwi-sull kunye nentsimi yengqolowa yenkohliso ye-Mycotoxins.