Iintsika ze-China Immunoaffinity ze-Aflatoxin Iyonke umzi-mveliso kunye nabavelisi

Iintsika ze-Immunoaffinity ze-Aflatoxin Iyonke yoMfanekiso oFakelweyo

Iikholamu ze-Immunoaffinity ze-Aflatoxin Iyonke

Inkcazelo emfutshane:

Iikholamu ze-AFT zisetyenziswa ngokudibanisa ne-HPLC, LC-MS, ikhithi yovavanyo ye-ELISA.

Inokuba luvavanyo lobungakanani be-AFB1, AFB2, AFG1, AFG2. Ilungele ukutya okuziinkozo, ukutya, amayeza aseTshayina, njl njl kwaye iphucula ukucoceka kweesampulu.

Thumela i-imeyile kuthi Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Iimpawu zemveliso

Inombolo yekati.	KH01102Z
Iipropati	Kuvavanyo lweAflatoxin Iyonke
Indawo yeMvelaphi	eBeijing, eTshayina
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 kwibhokisi nganye
Isampulu yosetyenziso	Ukutya, okuziinkozo, okuziinkozo kunye neziqholo
Ugcino	2-30℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Ubushushu begumbi

Izixhobo kunye neeReagents ezifunekayo

Izixhobo

Iireagents

Izixhobo

----Homogenizer ----Umxube weVortex

----Ibhotile yesampuli ----Isilinda yokulinganisa: 10ml, 100ml

----Iphepha lokucoca elisemgangathweni/I-Centrifuge -----Ibhalansi yohlalutyo (i-inductance: 0.01g)

----Uthweswe isidanga: 10ml ----Injector: 20ml

----Iflaski yeVolumetric: 250ml ----Ibhalbhu yerabha yepayipi yerabha

-----Micropipette: 100-1000ul ---- Ifaneli yeglasi 50ml

---- Izihluzi zeMicrofiber (Whatman, 934-AH, Φ11cm, 1.5um isangqa)

Iireagents

----Methanol (AR)

----Acetic acid (AR)

-----I-sodium chloride (NACL,AR)

---- Amanzi agayiweyo

Iinzuzo zemveliso

Kwinbon Inmmunoaffinity Columns zisebenzisa i-chromatography engamanzi ukwahlula, ukucocwa okanye uhlalutyo oluthile lwe-Aflatoxin Iyonke. Ngesiqhelo iikholamu zeKwinbon zidityaniswe neHPLC.
I-antibody ye-monoclonal ngokuchasene ne-Aflatoxin Iyonke inxulunyaniswe nemidiya edibanisayo kwikholamu. I-Mycotoxins kwisampulu iyakhutshwa, ihluzwe kwaye ixutywe. Yenza isisombululo sokutsalwa kwesampulu sidlula kwikholamu yeAflatoxin Iyonke. I-Aflatoxin (B1, B2, G1, G2) intsalela idityaniswe ne-antibody ngokwahlukileyo kwikholamu, isisombululo sokuhlamba sisuse ukungcola okungadityaniswanga. Okokugqibela, ukusebenzisa utywala be-methyl ukukhupha i-Aflatoxin B1, i-Aflatoxin B2, i-Aflatoxin G1, i-Aflatoxin G2.
Ngokuchaneka okuphezulu, iikholamu zeKwinbon AFT zinokubamba iimolekyuli ekujoliswe kuzo kwimeko ecocekileyo kakhulu. Kwakhona iikholamu zeKwinbon zihamba ngokukhawuleza, kulula ukusebenza. Ngoku isetyenziswa ngokukhawuleza nangokubanzi kwifidi nakwintsimi yeenkozo kwinkohliso ye-mycotoxins.