I-China i-tunoaffinity colomus colomus ye-Aflatoxin B1 Ifektri yeMveliso kunye nabaSebenzisi

Ikholamu -ffinity colomns ye-Aflatoxin B1

Inkcazo emfutshane:

I-Kwinbon Aflatoxin B1 Iikholamu zisetyenziswa ngokudibanisa ne-HPPC, lc-ms, ikhithi yovavanyo lwe-ELISA. Inokuba luvavanyo lwe-AFB1 ngenxa yeenkozo, iipiloti kunye neemveliso zabo, ioyile yemifuno kunye neemveliso ze-sne, i-souce ye-ne, isosi, iviniga, iziqholo, iziqholo, iziqholo kunye neti.

Thumela i-imeyile kuthi Imikhombandlela yokusetyenziswa

Iinkcukacha zemveliso

Iimpawu zeMveliso

Imveliso yemveliso

Ikati no.	Kh01104z
Iipropathi	Uvavanyo lwe-Aflatoxin B1
Indawo yemvelaphi	IBeijing, China
Igama lebrand	Kwinbon
Ubungakanani beyunithi	Iimvavanyo ezingama-25 ngebhokisi nganye
Isicelo seSampula	Iinkozo, iiperes kunye neemveliso zabo, ioyile yemifuno kunye namafutha, iimveliso zen, isosi, iviniga, amayeza e-China, i-Chinese
Ukugcinwa	2-30 ℃
Beka ubomi kwishelufa	Iinyanga ezili-12
Ukuhanjiswa	Igumbi le-temepe

Izixhobo kunye neRegednts eziyimfuneko

Izixhobo

Iirejista

Izixhobo

---- I-Homogenizer ---- Umxube we-PRTEX

---- Ibhotile yebhotile ---- isilinda sokulinganisa: 10ml, 100ml

---- Iphepha lokucoca ifiloti / i-Centrifuge --- ibhalansi ye-ANCHECT

---- I-Pipette ephumeleleyo: 10ml ---- i-Inactor: 20ml

---- I-dayilic flask: 250ml ---- i-raberte pipebette

---- i-micropipette: 100-1000ul ---- i-funnel ye-50ml

---- Iifilitha ze-microfiber (i-teman, i-934-Ah, φ11cm, 1.5um Isangqa)

Iirejista

---- i-metanol (i-AR)

---- i-acetic acid (i-AR)

---- I-sodium chloride (i-NACL, AR)

---- amanzi

Izibonelelo zeMveliso

I-Aflatoxin B1 ingcoliseko eqhelekileyo yokutya kubandakanya ii-peanuts, isidlo esinesidlo seCotton, MORN, kunye nezinye iingqolowa; kunye nokutya kwezilwanyana. I-Aflatoxin B1 ithathelwa ingqalelo eyona nto inetyhefu yetyhefu yetyhefu kwaye inxulunyaniswa kakhulu kwi-hepatocellial ye-shetoceallia (i-HCC) ebantwini.

I-Wikipedia icebisa ngeendlela ezichongiweyo;

I-thermat ye-chromatography
I-Enzyme-i-Insoncerbent Assay
Ikholamu yeKholamu yeKholamu ye-Fluorescence
Ikholamu yeKholamu yokuSebenzisa i-CHROMATOPY YOLWILE

I-Kwinbon Inmmmofnofinity yendlela yesithathu yendlela, isebenzise i-chromatography ye-chromatography yokwahlulahlula, ukucocwa okanye uhlalutyo oluthile lwe-Aflatoxin B1. Ngokwesiqhelo iikholamu zeKinbon zidityaniswa ne-HPPC.

Uhlalutyo lobungakanani be-HPPL ye-fungal toxins yindlela yokufumana iqonga. Zombini ezihamba phambili kunye nokubuyela umva kwi-chromatography yeSigaba isebenza. I-HLLC yeSigaba se-HPLC inoqoqosho, kulula ukusebenza, kwaye inobuthathaka obuphantsi. Uninzi lweetyhefu zinyibilikisi kwizigaba ze-polars ze-Colar kwaye zahlulwe yikholamu ye-chromatography, hlanganisa iimfuno zokufunyanwa ngokukhawuleza kweetyhubhu ezininzi zethayile. I-UPLC edibeneyo ngokuthe ngcembe isetyenziswa ngokuthe ngcembe, kunye neemodyuli zoxinzelelo oluphezulu kunye nobukhulu obuncinci kwaye zinokuphelisa isampulu yexesha elifanelekileyo, kwaye liphumelele isampulu yokwahlulahlula, kwaye iphumelele imvakalelo ephezulu.

Ngobunye obuphezulu, i-Kwinbon Aflatoxin B1 ikholamu inokubambisa iimolekyuli ezijolise kwimeko ecocekileyo. Iikholan zeKinbon ziyahamba ngokukhawuleza, i-EASSY ukuba isebenze. Ngoku kukhawuleze kwaye usebenzisa kakhulu kwi-sull kunye nentsimi yengqolowa yenkohliso ye-Mycotoxins.