imveliso

I-Chloramphenicol sisibulali-zintsholongwane esisebenzayo esibanzi, esisetyenziswa ngokuqhelekileyo kunyango lwezifo ezosulelayo zezilwanyana, kwaye sinefuthe elithintelayo kwiibhaktiriya ezininzi ze-pathogenic.Ingxaki enkulu ngeentsalela zekloramphenicol.I-Chloramphenicol inetyhefu enzulu kunye nemiphumo emibi, enokuthi ithintele umsebenzi we-hematopoietic womongo womntu, ibangela i-anemia ye-aplastic yabantu, i-leukocytosis ye-granular, i-neonatal, i-syndrome engwevu ngaphambi kwexesha kunye nezinye izifo, kunye nokugxila okuphantsi kweentsalela zamachiza kunokubangela izifo.Ke ngoko, iintsalela ze-chloramphenicol ekutyeni kwezilwanyana zibeka isoyikiso esikhulu kwimpilo yabantu.Ke ngoko, iye yavalwa okanye isetyenziswa ngokungqongqo kwi-EU nase-US.

Kwinbon le khithi yimveliso entsha esekwe kwi-ELISA, ekhawulezayo (kuphela i-50min kwintsebenzo enye), kulula, ichanekile kwaye inovakalelo xa kuthelekiswa nohlalutyo lwezixhobo eziqhelekileyo, kwaye ke inokunciphisa kakhulu impazamo yokusebenza kunye nokuqina komsebenzi.

Iimpendulo ezinqamlezayo

I-Chloramphenicol……………………………………..……100%

I-Chloramphenicol palmitate………………………………<0.1%

IThiamphenicol…………………………………..……..<0.1%

Florfenicol………………………………………………<0.1%

I-Cetofenicol…………………………………………………<0.1%

Izixhobo zeKit

Ipleyiti yeMicrotiter eqatywe nge-antigen, ii-96wells

Izisombululo eziqhelekileyo (6×1ml/ibhotile)

0ppb, 0.025ppb, 0.075ppb, 0.3ppb, 1.2ppb, 4.8ppb

Isisombululo esisemgangathweni: (1ml/ibhotile) …….…100ppb

I-enzyme egxininisiweyo idibanisa 1ml….…..……………..….…..….…..….……….

I-Enzyme conjugate diluent 10ml….…..….…………………..…..….…..….…..…..umnqwazi ocacileyo

Isisombululo A 7ml ……………………………………………… ..............….………….ikepusi emhlophe

Isisombululo B 7ml……………………………………………………… .........………………….ikepusi ebomvu

Misa isisombululo 7ml ……………………………………………… ........…….…….…..ikepusi etyheli

20×Isisombululo sokuhlamba esigxininisiweyo 40ml………………………………………….….

2×Isisombululo esigxininisiweyo sokutsalwa kwe-50ml ............................................ ...........ikepusi eluhlaza

Iziphumo

1 Ipesenti yokuthatha

Amaxabiso aphakathi amaxabiso e-absorbence afunyenwe kwimigangatho kunye neesampuli zihlulwe ngexabiso lokuthatha umgangatho wokuqala (umgangatho we-zero) kwaye zanda nge-100%.Umgangatho we-zero wenziwa ulingane ne-100% kwaye amaxabiso e-absorpsor acatshulwe ngokweepesenti.

B ——umgangatho wokungabikho (okanye isampuli)

B₀ —-absorbance zero umgangatho

2 Igophe eliMgangatho

Ukuzoba igophe elisemgangathweni: thatha ixabiso lemigangatho njenge-y-axis, i-semi logarithmic yoxinaniso lwesisombululo semigangatho yeCAP (ppb) njenge-x-axis.

Ugxininiso lwe-CAP lwesampulu nganye (ppb), enokufundwa kwi-curve yokulinganisa, iphinda iphindwe yi-Dilution factor ehambelanayo yesampuli nganye elandelwayo, kwaye i-concentration yangempela yesampuli ifunyenwe.

Nceda uqaphele:

Uhlalutyo lwedatha yeekhithi ze-ELISA, isoftware ekhethekileyo iye yaphuhliswa, enokuthi i-odolwe ngesicelo.