Elisa Test Kit of AOZ
Malunga
Le kit ingasetyenziselwa uhlalutyo lobungakanani kunye nomgangatho we-AOZ intsalela kwizicubu zezilwanyana (inkukhu, inkomo, ihagu, njl njl), ubisi , ubusi kunye namaqanda.
Uhlalutyo lwentsalela yamachiza e-nitrofuran kufuneka lusekelwe ekufumaneni i-metabolites ebophelelwe kwizicubu ze-nitrofuran iziyobisi zabazali, ezibandakanya i-Furazolidone metabolite (AOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) kunye ne-Nitrofurazone metabolite (SEM).
Xa kuthelekiswa neendlela zechromatographic, ikhithi yethu ibonisa inzuzo enkulu malunga nobuntununtunu, umda wokubona, izixhobo zobugcisa kunye nexesha elifunekayo.
Izixhobo zeKit
• Ipleyiti yeMicrotiter eqatywe nge-antigen, amaqula angama-96
• Izisombululo eziqhelekileyo(iibhotile ezi-6,1ml/ibhotile)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
• Ulawulo olusemgangathweni lwe-spiking : (1ml/ibhotile)..............................................….100ppb
• Enzyme conjugate concentrate 1.5ml.............................................….….ikepusi ebomvu
• Isisombululo se-antibody sigxininiswe 0.8ml………………………………....…ikepusi eluhlaza
• I-Substrate A 7ml…………………..........................................……………..ikepusi emhlophe
• I-Substrate B7ml………………………………………….........................................................…..ikepusi ebomvu
• Misa isisombululo 7ml………………………………………………….………ikepusi etyheli
• 20×isisombululo esigxininisiweyo sokuhlamba 40ml ………………………….……icap ecacileyo
• 2×isisombululo esigxininisiweyo sotsalo 60ml……………………..…………….ikepusi eluhlaza
• 2-Nitrobenzaldehyde 15.1mg……………………………………………………ikepusi emnyama
Uvakalelo, ukuchaneka kunye nokuchaneka
Uvakalelo: 0.025ppb
Umda wokufunyanwa………………..………………………………………….1ppb
Ukuchaneka:
Izicubu zezilwanyana (izihlunu nesibindi)……….……………75±15%
Ubusi……………………………………………………..90±20%
Iqanda……………………………………………………..…90±20%
Ubisi……………………………………………………..…90±10%
Ukuchaneka:I-CV yekhithi ye-ELISA ingaphantsi kwe-10%.
Inqanaba lokuwela
I-Furazolidone metabolite (AOZ)……………………………………………..100%
I-Furaltadone metabolite (AMOZ)…………………………………………<0.1%
I-Nitrofurantoin metabolite (AHD)………………………………………<0.1%
I-Nitrofurazone metabolite (SEM)…………………………………………<0.1%
IFurazolidone……………………………………………………….…..…16.3%
Furaltadone………………………………………………………….…<1%
INitrofurantoin…………………………………………………….….…<1%
INitrofurazone…………………………………………………………..…<1%