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Elisa Test Kit of AMOZ Featured Image
  • Elisa Test Kit of AMOZ
  • Elisa Test Kit of AMOZ

Elisa Test Kit of AMOZ

Inkcazelo emfutshane:

Le khithi ingasetyenziselwa uhlalutyo lobungakanani kunye nomgangatho wentsalela ye-AMOZ kwiimveliso zasemanzini (intlanzi kunye ne-shrimp), njl. Uvavanyo lwe-Enzyme immunoassays, xa kuthelekiswa neendlela zechromatographic, lubonisa iingenelo ezinkulu malunga nobuntununtunu, umda wokufumanisa, izixhobo zobugcisa kunye nexesha elifunekayo.
Le kit yenzelwe ukufumanisa i-AMOZ ngokusekelwe kumgaqo-nkqubo wokukhuphisana ngokungangqalanga kwe-enzyme immunoassay.Imingxuma emincinci igqunywe nge-capture BSA edityanisiweyo
i-antigen.I-AMOZ kwisampulu ikhuphisana ne-antigen egqunywe kwipleyiti ye-microtiter ye-antibody eyongeziweyo.Emva kokongezwa kwe-enzyme conjugate, i-substrate ye-chromogenic isetyenziswa kwaye umqondiso ulinganiswa nge-spectrophotometer.Ukufunxa kuhambelana ngokuphambeneyo noxinaniso lwe-AM OZ kwisampulu.


Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

I-2.Izidakamizwa ze-nitrofuran i-furaltadone, i-nitrofurantoin kunye ne-nitrofurazone ziye zavalwa ukusetyenziswa kwimveliso yezilwanyana zokutya kwi-EU kwi-1993, kwaye ukusetyenziswa kwe-furazolidone kwakungavunyelwe kwi-1995. I-metabolites ebophelelweyo yeziyobisi zabazali be-nitrofuran, ekubeni iziyobisi zabazali zixutywe ngokukhawuleza, kwaye i-nitrofuran ebophelelwe kwi-metabolites iya kugcina ixesha elide, ngoko ke i-metabolites isetyenziswa njengento ekujoliswe kuyo ekubhaqweni kokusetyenziswa kakubi kwe-nitrofurans.I-Furazolidone metabolite (AMOZ), i-Furaltadone metabolite (AMOZ), i-Nitrofurantoin metabolite (AHD) kunye ne-Nitrofurazone metabolite (SEM).

Iinkcukacha

1.Elisa Test Kit of AMOZ

2.Ikati.KA00205H-96 Wells

3.Izixhobo zeKit
● Icwecwe leMicrotiter elinamaqula angama-96 aqatywe ngeantigen
● Izisombululo eziqhelekileyo (iibhotile ezi-6)
0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb
● Isisombululo esisemgangathweni: (1ml/ibhotile) ………………………………………………….100ppb
● I-Enzyme conjugate 1ml………………………………………………………..…………….ikhephu ebomvu
● Isisombululo se-antibody 7ml …………………………………………………..………….….ikepusi eluhlaza
● isisombululo A 7ml ……………………………………………………………………………
● isisombululo B 7ml…………………………………………………………….………..………
● Stop solution 7ml ……………………………………………………….
● 20×isisombululo esigxininisiweyo sokuhlamba 40ml……………………………………….……
● 2×isisombululo esigxininisiweyo sotsalo 50ml
● 2-Nitrobenzaldehyde 15.1mg………………………………………………………….…

4.Uvakalelo, ukuchaneka nokuchaneka
Uvakalelo: 0.05ppb
Umda wokufunyanwa
Iimveliso zasemanzini(intlanzi kunye nemfanzi)………….………… 0.1ppb
Ukuchaneka
Iimveliso zasemanzini(intlanzi kunye nemfanzi)….……………… 95±25%
Ukuchaneka: I-CV yekhithi ye-ELISA ingaphantsi kwe-10%.

5.Ireyithi enqamlezileyo
I-Furaltadone metabolite(AMOZ)…………………………………………………100%
I-Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%
Nitrofurantoin metabolite(AHD)………………………………………………<0.1%
I-Nitrofurazone metabolite(SEM)…………………………………………..…<0.1%
Furaltadone…………………………………………………………….…….11.1%
IFurazolidone……………………………………………………….….…..…<0.1%
INitrofurantoin……………………………………………………….….…<1%
INitrofurazone………………………………………………………………..…<1%

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