Elisa Test Kit of AMOZ
Malunga
Le khithi ingasetyenziselwa uhlalutyo lobungakanani kunye nomgangatho wentsalela ye-AMOZ kwiimveliso zasemanzini (intlanzi kunye ne-shrimp), njl. Uvavanyo lwe-Enzyme immunoassays, xa kuthelekiswa neendlela zechromatographic, lubonisa iingenelo ezinkulu malunga nobuntununtunu, umda wokufumanisa, izixhobo zobugcisa kunye nexesha elifunekayo.
Le kit yenzelwe ukufumanisa i-AMOZ ngokusekelwe kumgaqo-nkqubo wokukhuphisana ngokungangqalanga kwe-enzyme immunoassay.Imingxuma emincinci igqunywe nge-capture BSA edityanisiweyo
i-antigen.I-AMOZ kwisampulu ikhuphisana ne-antigen egqunywe kwipleyiti ye-microtiter ye-antibody eyongeziweyo.Emva kokongezwa kwe-enzyme conjugate, i-substrate ye-chromogenic isetyenziswa kwaye umqondiso ulinganiswa nge-spectrophotometer.Ukufunxa kuhambelana ngokuphambeneyo noxinaniso lwe-AM OZ kwisampulu.
Izixhobo zeKit
·I-Microtiter plate enamaqula angama-96 aqatywe nge-antigen
Izisombululo eziqhelekileyo(iibhotile ezi-6)
0ppb, 0.05ppb, 0.15ppb, 0.45ppb, 1.35ppb, 4.05ppb
·Isisombululo esisemgangathweni: (1ml/ibhotile) …………………………………………………100ppb
·I-Enzyme conjugate 1ml………………………………………………………………………….icap ebomvu
·Isisombululo se-Antibody 7ml …………………………………………………..…………….….ikepusi eluhlaza
·Isisombululo A 7ml……………………………………………………….…………………ikepusi emhlophe
·Isisombululo B 7ml…………………………………………………………….………..…… ikepusi ebomvu
·stop solution 7ml ……………………………………………………….…………ikepusi etyheli
·20×isisombululo esigxininisiweyo sokuhlamba 40ml……………………………………….……
·2×isisombululo esigxininisiweyo sokutsalwa 50ml………………………………………….…….
·2-Nitrobenzaldehyde 15.1mg……………………………………………………………………
Uvakalelo, ukuchaneka kunye nokuchaneka
Uvakalelo: 0.05ppb
Umda wokufunyanwa
Iimveliso zasemanzini(intlanzi kunye nemfanzi)………….………… 0.1ppb
Ukuchaneka
Iimveliso zasemanzini(intlanzi kunye nemfanzi)….……………… 95±25%
Ukuchaneka:I-CV yekhithi ye-ELISA ingaphantsi kwe-10%.
Inqanaba lokuwela
I-Furaltadone metabolite(AMOZ)…………………………………….…………100%
I-Furazolidone metabolite(AMOZ)……………………………..………………..<0.1%
Nitrofurantoin metabolite(AHD)………………………………….…………<0.1%
I-Nitrofurazone metabolite(SEM)……………………………………………..…<0.1%
Furaltadone……………………………………………………………….…….11.1%
I-Furazolidone………………………………………………………….…...…<0.1%
INitrofurantoin……………………………………………………….….…<1%
INitrofurazone………………………………………………….………………..…<1%