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Elisa Test Kit of Aflatoxin B1 Umfanekiso obonakalayo
  • Elisa Ikhithi yoVavanyo ye-Aflatoxin B1
  • Elisa Ikhithi yoVavanyo ye-Aflatoxin B1
  • Elisa Ikhithi yoVavanyo ye-Aflatoxin B1

Elisa Ikhithi yoVavanyo ye-Aflatoxin B1

Inkcazelo emfutshane:

Iidosi ezinkulu ze-aflatoxins zikhokelela kwityhefu ebukhali (i-aflatoxicosis) enokubeka ubomi esichengeni, ngokwesiqhelo ngomonakalo esibindini.

I-Aflatoxin B1 yi-aflatoxin eveliswa yi-Aspergillus flavus kunye ne-A. parasiticus.Yi-carcinogen enamandla kakhulu.La mandla e-carcinogenic ayohluka kwiintlobo ngeentlobo nezinye, ezinje ngeempuku kunye neenkawu, zibonakala zichaphazeleka ngakumbi kunezinye.I-Aflatoxin B1 yingcoliseko eqhelekileyo kwiindidi zokutya okuquka amandongomane, i-cottonseed meal, umbona, kunye nezinye iinkozo;ngokunjalo nokutya kwezilwanyana.I-Aflatoxin B1 ithathwa njengeyona ityhefu ye-aflatoxin kwaye ibandakanyeka kakhulu kwi-hepatocellular carcinoma (HCC) ebantwini.[cating] Kwizilwanyana, i-aflatoxin B1 ikwabonakaliswe njenge-mutagenic, i-teratogenic, kwaye ibangela ukugonywa komzimba.Iisampulu ezininzi kunye neendlela zohlalutyo ezibandakanya i-chromatography (TLC), i-high-performance liquid chromatography (HPLC), i-mass spectrometry, kunye ne-enzyme-linked immunosorbent assay (ELISA), phakathi kwezinye, zisetyenziselwe ukuvavanya ukungcoliseka kwe-aflatoxin B1 ekutyeni. .Ngokutsho kwe-Food and Agriculture Organisation (FAO), amanqanaba aphezulu anyanyezelwayo e-aflatoxin B1 emhlabeni wonke axelwe ukuba akuluhlu lwe-1-20 μg/kg ekutyeni, kunye ne-5-50 μg/kg kwisondlo senkomo esityayo ngo-2003.


Imikhomba-ndlela yokusetyenziswa

Iinkcukacha zeMveliso

Iithegi zeMveliso

Malunga

Le khithi ingasetyenziselwa uhlalutyo lomgangatho kunye nobungakanani be-aflatoxin B1 kwi-oyile etyiwayo, i-peanut, iinkozo ze-cereal, isosi yesoya, iviniga kunye nokutya (ifidi ekrwada, imathiriyeli yebhetshi exutyiweyo kunye nemathiriyeli egxininisiweyo). uhlalutyo lwezixhobo.

Le mveliso isekelwe kukhuphiswano olungathanga ngqo lwe-ELISA, olukhawulezayo, oluchanekileyo kunye nobuthathaka xa kuthelekiswa nohlalutyo lwezixhobo eziqhelekileyo.Ifuna kuphela i-45min kumsebenzi omnye, onokunciphisa kakhulu impazamo yokusebenza kunye nokuqina komsebenzi.

Amacandelo ekhithi

• I-Microtiter plateprecoated ne-antigen, amaqula angama-96

• Isisombululo esisemgangathweni ×6ibhotile(1ml/ibhotile)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• I-Enzyme conjugate 7ml……………………………………………………………………………

•Isisombululo se-antibody7ml................................................................. ......................………ikepusi eluhlaza

• I-Substrate A 7ml……………………………………………………………….…………………ikepusi emhlophe

• I Substrate B 7ml……………….…………………………………………………….…………ikepusi ebomvu

• Misa isisombululo 7ml……….…………………………………………………………..………ikepusi etyheli

• 20×isisombululo esigxininisiweyo sokuhlamba 40ml …………………………………………………

• 2×isisombululo esigxininisiweyo sotsalo 50ml…………………………………………………………

Uvakalelo, ukuchaneka kunye nokuchaneka

Uvakalelo:0.05ppb

Umda wokufunyanwa

Isampulu yeoli etyiwayo.............................................. ........................................................................0.1ppb

Peanut................................................. .................................................. .......................0.2ppb

Icereal................................................. .................................................. .....................0.05ppb

Ukuchaneka

Isampulu yeoli etyiwayo.............................................. ....................................................................80±15%

Peanut................................................. .................................................. ....................80±15%

Icereal................................................. .................................................. ....................80±15%

Ukuchaneka

Ukwahluka komlinganiso wekhithi ye-ELISA ingaphantsi kwe-10%.

Inqanaba lokuwela

I-Aflatoxin B1·················100%

I-Aflatoxin B2 ·····················81 .3%

I-Aflatoxin G1 · ·····················62%

I-Aflatoxin G2 · ·····················22.3%

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