Aflatoksin B1 uchun Elisa test to'plami
Aflatoksinlarning katta dozalari hayot uchun xavfli bo'lishi mumkin bo'lgan o'tkir zaharlanishga (aflatoksikoz) olib keladi, odatda jigarning shikastlanishi.
Aflatoksin B1 Aspergillus flavus va A. parasiticus tomonidan ishlab chiqarilgan aflatoksindir.Bu juda kuchli kanserogen.Ushbu kanserogen ta'sir turlarga qarab farq qiladi, ba'zilari, masalan, kalamushlar va maymunlar boshqalarga qaraganda ancha sezgir.Aflatoksin B1 yeryong'oq, paxta chigiti, makkajo'xori va boshqa donlar kabi turli xil oziq-ovqatlarda keng tarqalgan ifloslantiruvchi hisoblanadi;shuningdek, hayvonlarning ozuqalari.Aflatoksin B1 eng zaharli aflatoksin hisoblanadi va u odamlarda gepatotsellyulyar karsinomaga (HCC) katta ta'sir ko'rsatadi.[iqtibos keltirish kerak] Hayvonlarda aflatoksin B1 mutagen, teratogen va immunosupressiyaga sabab bo'lishi ham ko'rsatilgan.Oziq-ovqatlarda aflatoksin B1 kontaminatsiyasini tekshirish uchun bir qancha namuna olish va analitik usullar, jumladan yupqa qatlamli xromatografiya (TLC), yuqori samarali suyuqlik xromatografiyasi (HPLC), massa spektrometriyasi va fermentga bog'liq immunosorbent tahlili (ELISA) qo'llanilgan. .Oziq-ovqat va qishloq xo'jaligi tashkiloti (FAO) ma'lumotlariga ko'ra, 2003 yilda butun dunyo bo'ylab aflatoksin B1 ning maksimal ruxsat etilgan darajasi oziq-ovqat mahsulotida 1-20 mkg / kg va oziq-ovqatda 5-50 mkg / kg oralig'ida bo'lgan.
Tafsilotlar
1.Aflatoksin B1 uchun Elisa test to'plami
2.Mushuk.KA07202H-96 quduqlari
3. To'plam komponentlari
● Antijen bilan oldindan qoplangan mikrotitrli plastinka, 96 ta quduq
● Standart eritma × 6 shisha (1 ml/shisha)
0ppb, 0,02ppb, 0,06ppb, 0,18ppb, 0,54ppb,1,62ppb
● Enzim konjugati 7ml……………………………………………………………………………………………………………
● Antikor eritmasi 7 ml................................................. ................................................................ yashil qalpoq
● Substrat A 7ml……………………………………………………….………………oq qopqoq
● Substrat B 7ml…………….…………………………………………………………………………………………………………………
● STOP STOP QOIM 7ML ........................................... Sariq qopqoq
● 20 × konsentrlangan yuvish eritmasi 40 ml ……………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………… ● 40ml 40ml.
● 2 × konsentrlangan ekstraksiya eritmasi 50ml………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………… '''liksiz
4.Sezuvchanlik, aniqlik va aniqlik
Sezuvchanlik: 0,05ppb
5.Aniqlash chegarasi
Oziq-ovqat yog'i namunasi................................................. ................................................................ ......................0,1ppb
Yeryong'oq................................................. ................................................................ ...................0,2ppb
Don................................................................. ................................................................ .................0,05ppb
Aniqlik
Oziq-ovqat yog'i namunasi................................................. ................................................................ ................80±15%
Yeryong'oq................................................. ................................................................ ................80±15%
Don................................................................. ................................................................ ................80±15%
Aniqlik:ELISA to'plamining o'zgaruvchanlik koeffitsienti 10% dan kam.
6. Kross-stavka
Aflatoksin B1··················100%
Aflatoksin B2·······················81 ,3%
Aflatoksin G1·······················62%
Aflatoksin G2························22,3%
