Aflatoksin B1 uchun Elisa test to'plami
Haqida
Ushbu to'plamdan oziq-ovqat yog'i, yeryong'oq, don donalari, soya sousi, sirka va ozuqa (xom yem, aralash partiyalar va konsentrlangan materiallar) tarkibidagi aflatoksin B1 ni sifat va miqdoriy tahlil qilish uchun foydalanish mumkin.. instrumental tahlil.
Ushbu mahsulot an'anaviy instrumental tahlil bilan solishtirganda tezkor, aniq va sezgir bo'lgan bilvosita raqobatbardosh Elishayga asoslangan.Bitta operatsiyaga atigi 45 daqiqa kerak bo'ladi, bu operatsiya xatosini va ish intensivligini sezilarli darajada kamaytiradi.
To'plam komponentlari
• Antigen bilan oldindan qoplangan mikrotitr plastinka, 96 ta quduq
• Standart eritma × 6 shisha (1 ml/shisha)
0ppb, 0,02ppb, 0,06ppb, 0,18ppb, 0,54ppb,1,62ppb
• Ferment konjugati 7ml………………………………………………………………………………………………………………
• Antikor eritmasi7ml............................................................................. .................………yashil qalpoq
• Substrat A 7ml………………………………………………….……………… oq qalpoq
• Substrat B 7ml……………….…………………………………………….………… qizil qalpoq
• Stop eritmasi 7ml……….…….…………………………………………….………sariq qalpoqli
• 20× konsentrlangan yuvish eritmasi 40ml ………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….
• 2 × konsentratsiyalangan ekstraktsiya eritmasi 50mll ...................................
Sezuvchanlik, aniqlik va aniqlik
Sezuvchanlik:0,05 ppb
Aniqlash chegarasi
Oziq-ovqat yog'i namunasi................................................. ..............................................................................0,1ppb
Yeryong'oq................................................. ................................................................ ...................0,2ppb
Don................................................................. ................................................................ .................0,05ppb
Aniqlik
Oziq-ovqat yog'i namunasi................................................. ......................................................................80±15%
Yeryong'oq................................................. ................................................................ ................80±15%
Don................................................................. ................................................................ ................80±15%
Aniqlik
ELISA to'plamining o'zgaruvchanlik koeffitsienti 10% dan kam.
Kross-stavka
Aflatoksin B1·················100%
Aflatoksin B2······················· 81 .3%
Aflatoksin G1·······················62%
Aflatoksin G2························22,3%