AOZ-yň ELisa synag toplumy
Takmynan
Bu toplum haýwan dokumalarynda (towuk, mal, doňuz we ş.m.), süýt, bal we ýumurtgalarda AOZ galyndysynyň mukdar we hil derňewinde ulanylyp bilner.
Nitrofuran dermanlarynyň galyndysynyň derňewi, Furazolidon metabolitini (AOZ), Furaltadon metabolitini (AMOZ), Nitrofurantoin metabolitini (AHD) we Nitrofurazon metabolitini (SEM) öz içine alýan nitrofuran ene dermanlarynyň dokuma bagly metabolitlerini kesgitlemäge esaslanmalydyr.
Hromatografiki usullar bilen deňeşdirilende, toplumymyz duýgurlyk, kesgitleme çäkleri, tehniki enjamlar we wagt talaplary babatynda ep-esli artykmaçlyklary görkezýär.
Kit komponentleri
• Antigen bilen örtülen mikrotiter plastinka, 96 guýy
• Adaty çözgütler (6 çüýşe, 1ml / çüýşe)
0ppb, 0.025ppb, 0.075ppb, 0.225ppb, 0.675ppb, 2.025ppb
• Adaty dolandyryş: (1ml / çüýşe) ................................................….100ppb
• Ferment konýugat konsentraty 1,5ml ..............................................…
• Antikor ergini 0.8ml jemlenendir ……………………………....… Capaşyl gapak
• 7ml substrat ……… .........................................................… ....… ..… .. ak gapak
• B7ml substrat ……… ..............… .................................................… ..… .. gapak
• 7ml çözgüdi duruzyň …………………………………….……… sary gapak
• 20 × konsentrirlenen ýuwujy ergin 40ml ………………………. …… aç-açan gapak
• 2 × konsentrirlenen çykaryş ergini 60ml …………………..……………
• 2-Nitrobenzaldegid 15.1mg ……………………… gara gapak
Duýgurlyk, takyklyk we takyklyk
Duýgurlyk: 0.025ppb
Ectionüze çykarmak çägi…………… .. ………………………… 0.1ppb
Takyklyk:
Haýwan dokumasy (myşsa we bagyr) ………. ………… 75 ± 15%
Bal ……………………………… .. ……………… ..90 ± 20%
Gumurtga …………………………… .. …………… ..… 90 ± 20%
Süýt …………………………… .. …………… ..… 90 ± 10%
Takyklyk:ELISA toplumynyň CV-i 10% -den az.
Kesgitleme nyrhy
Furazolidon metaboliti (AOZ) …………………………. …………… ..100%
Furaltadon metaboliti (AMOZ) …………………………. …………… <0,1%
Nitrofurantoin metaboliti (AHD) ……………………………………… <0,1%
Nitrofurazon metaboliti (SEM) ……………………. …………… ...… <0,1%
Furazolidon ……………………………….… ..… 16.3%
Furaltadon ……………………….… <1%
Nitrofurantoin ……………………………. …….… <1%
Nitrofurazon ………………………… ..… <1%