Aflatoksin B1-iň Elisa synag toplumy
Takmynan
Bu toplum iýilýän ýagda, nohutda, däneli dänelerde, soýa sousunda, sirkede we iýmitde (çig iýmit, garyşyk partiýa materiallary we konsentrirlenen materiallar) aflatoksin B1-i hil we mukdar taýdan derňemek üçin ulanylyp bilner.. gurallaryň derňewi.
Bu önüm adaty gural derňewi bilen deňeşdirilende çalt, takyk we duýgur gytaklaýyn bäsdeşlik ELISA esaslanýar.Bir amalda bary-ýogy 45min gerek, bu operasiýa ýalňyşlygyny we iş güýjüni ep-esli azaldyp biler.
Kit komponentleri
• Antigen, 96 guýy bilen örtülen mikrotiter
• Standart çözgüt × 6 çüýşe (1ml / çüýşe)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
• Ferment konýugaty 7ml ………………… gyzyl gapak
• Antikor çözgüdi7ml ................................................................. ................. ……… ýaşyl gapak
• 7ml substrat ………………………………………….………… ...… ak gapak
• B 7ml substrat ………………. ………………………………………….………… gyzyl gapak
• 7ml çözgüdi bes ediň ………. ……. ………………………………………….. ……… sary gapak
• 20 × konsentrirlenen ýuwujy ergin 40ml ……………………………………
• 2 × konsentrirlenen çykarmak ergini 50ml ………………………………………… gök gapak
Duýgurlyk, takyklyk we takyklyk
Duýgurlyk:0.05ppb
Ectionüze çykarmak çägi
Iýilýän ýag nusgasy ............................................... .......................................................................... 0.1ppb
Arahis ................................................. .................................................. ....................... 0.2ppb
Galla ................................................. .................................................. ...................... 0.05ppb
Takyklyk
Iýilýän ýag nusgasy ............................................... ...................................................................... 80 ± 15%
Arahis ................................................. .................................................. ..................... 80 ± 15%
Galla ................................................. .................................................. ..................... 80 ± 15%
Takyklyk
ELISA toplumynyň üýtgeýiş koeffisiýenti 10% -den az.
Kesgitleme nyrhy
Aflatoksin B.1· 100% 100%
Aflatoksin B2 ··················· 81 .3%
Aflatoksin G1 ··············%
Aflatoksin G2 ················· 22.3%