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Dosis aflatoksin anu ageung ngakibatkeun karacunan akut (aflatoxicosis) anu tiasa ngancem kahirupan, biasana ngalangkungan karusakan ati.
Aflatoksin B1 mangrupa aflatoksin dihasilkeun ku Aspergillus flavus jeung A. parasiticus.Éta karsinogén pisan potent.Poténsi karsinogenik ieu béda-béda dina sababaraha spésiés, sapertos beurit sareng monyét, sigana langkung rentan tibatan anu sanés.Aflatoxin B1 mangrupakeun contaminant umum dina rupa-rupa pangan kaasup kacang, cottonseed tepung, jagong, jeung séréal séjén;ogé pakan ternak.Aflatoksin B1 dianggap aflatoksin anu paling beracun sareng seueur implikasi dina karsinoma hépatoselular (HCC) dina manusa.[rujukan?] Dina sasatoan, aflatoksin B1 ogé parantos kabuktian mutagenik, teratogenik, sareng nyababkeun imunosupresi.Sababaraha metode sampling sareng analitik kalebet kromatografi lapis tipis (TLC), kromatografi cair kinerja tinggi (HPLC), spéktrométri massa, sareng uji immunosorbent-enzim-linked (ELISA), diantarana, parantos dianggo pikeun nguji kontaminasi aflatoksin B1 dina pangan. .Numutkeun kana Organisasi Pangan sareng Tatanén (FAO), tingkat aflatoksin B1 anu ditoleransi maksimal di dunya dilaporkeun dina kisaran 1-20 μg / kg dina dahareun, sareng 5-50 μg / kg dina pakan ternak dina taun 2003.

Rincian

1.Elisa Test Kit pikeun Aflatoxin B1

2. Ucing.KA07202H-96sumur

3. komponén Kit
● Microtiter plateprecoated kalawan antigen, 96 sumur
● Solusi Standar ×6botol(1ml/botol)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Énzim konjugat 7ml…………………………………………………………………………………………………………
● Solusi antibodi7ml................................................................ ......................................................cap héjo
● Substrat A 7ml……………………………………………………………………………………………………
● Substrat B 7ml………………………………………………………………………………………………………………
● Eureun solusi 7ml…………………………………………………………………………………………..…….. tutup koneng
● 20 × solusi nyeuseuh kentel 40ml …………………………………………cap transparan
● 2×leyuran ékstraksi kentel 50ml………………………………………………cap biru

4.Sensitipitas, akurasi sarta precision
Sensitipitas: 0.05ppb

5.Batesan deteksi
Sampel minyak nabati ................................................................. ................................................ ........................0.1ppb
Kacang................................................. ................................................ .......................0.2ppb
Sereal................................................. ................................................ ......................0.05ppb
Akurasi
Sampel minyak nabati ................................................................. ................................................... .....................80±15%
Kacang................................................. ................................................ .....................80±15%
Sereal................................................. ................................................... .....................80±15%
Precision:Koéfisién variasi kit ELISA kirang ti 10%.

6. Laju Palang
Aflatoksin B1················· 100%
Aflatoksin B2························· 81 ,3%
Aflatoksin G1······················· 62%
Aflatoksin G2························ 22,3%