China Litšiea tsa Immunoaffinity bakeng sa fektheri ea ho lemoha Fumonisin le bahlahisi

Litšiea tsa Immunoaffinity bakeng sa phumano ea Fumonisin Setšoantšo se bontšitsoeng

Litšiea tsa Immunoaffinity bakeng sa ho fumanoa ha Fumonisin

Tlhaloso e Khutšoanyane:

Likholomo tsa Kwinbon Fumonisin li sebelisoa ka ho kopanngoa le HPLC, LC-MS, ELISA test kit. E loketse lijo-thollo, lijo, meriana ea Sechaena, joalo-joalo mme e ntlafatsa bohloeki ba lisampole.

Re romelle lengolo-tsoibila Litaelo Bakeng sa Tšebeliso

Lintlha tsa Sehlahisoa

Li-tag tsa Sehlahisoa

Litlhaloso tsa sehlahisoa

Nomoro ea katse.	KH01001Z
Thepa	Bakeng saFumonisintekolo
Sebaka sa Tšimoloho	Beijing, China
Lebitso la Brand	Kwinbon
Boholo ba Yuniti	Liteko tse 25 ka lebokose le leng
Sesebelisoa sa Mohlala	Fepa, lijo-thollo, lijo-thollo le linoko
Polokelo	2-30 ℃
Shelf-bophelo	Likhoeli tse 12
Phano	Mocheso oa kamore

Thepa & Reagents Ho Hlokahala

Thepa

Li-reagents

Thepa

---- Homogenizer ---- Vortex mixer

----Botlolo ea mohlala ----Silindara ea ho lekanya: 10ml, 100ml

----Pampiri ea sesefa ea boleng bo botle/Centrifuge -----Analytical balance (inductance: 0.01g)

-----Pipette e alohileng mangolo: 10ml ----Injector: 20ml

---- Botlolo ea Volumetric: 250ml ----Lebone la peipi ea rabara

----Micropipette: 100-1000ul ---- Funnel ea khalase 50ml

----Masefe a Microfiber (Whatman, 934-AH, Φ11cm, selikalikoe sa 1.5um)

Li-reagents

---- Methanol (AR)

----Acetic acid (AR)

---- sodium chloride (NACL,AR)

-----Metsi a hloekisitsoeng

Melemo ea lihlahisoa

Fomonisin(FB) ke mofuta oa metabolite e qhibilihang ka metsing e hlahisoang ke li-fungus tse chefo. Haholo-holo e silafatsa lijo-thollo le lihlahisoa tsa tsona, 'me e na le chefo e matla le kotsi e ka bang teng ea kankere ho mehlape e itseng, 'me e fetohile sebaka se seng sa lipatlisiso ka mor'a aflatoxin. E ka baka tšenyo e kholo ho batho le liphoofolong hore ntho eo e hlahisoa ka bongata pooneng le lihlahisoa tse ling tsa temo lefatshe.

Wikipedia e khothalletsa mekhoa e latelang ea ho lemoha;

chromatography e tšesaane,
Teko ea immunosorbent e amanang le enzyme
Immunoaffinity column fluorescence
Immunoaffinity column high performance liquid chromatography

Kwinbon Inmmunoaffinity Columns ke mokhoa oa boraro, o sebelisa chromatography ea metsi bakeng sa karohano, tlhoekiso kapa tlhahlobo e khethehileng ea Fumonisin. Hangata likholomo tsa Kwinbon li kopantsoe le HPLC.

Tlhahlobo ea palo ea HPLC ea chefo ea fungal ke mokhoa oa ho lemoha o holileng. Ka bobeli chromatography ea mohato oa pele le oa morao oa sebetsa. Reverse phase HPLC ke ea moruo, e bonolo ho sebetsa, 'me e na le chefo e tlase ea solvent. Bongata ba chefo bo qhibiliha ka mekhahlelo ea polar mobile ebe bo aroloa ke litšiea tse sa polar chromatography, tse fihlelang litlhoko tsa ho lemoha ka potlako chefo ea fungal e mengata ea sampole ea lebese. Li-detectors tse kopantsoeng tsa UPLC li ntse li sebelisoa butle-butle, ka li-module tse phahameng tsa khatello le boholo bo nyane le likholomo tsa chromatography, tse ka khutsufatsang nako ea ho sebetsa, ho ntlafatsa katleho ea karohano ea chromatographic, le ho finyella kutloisiso e phahameng.

Ka boits'oaro bo phahameng, litšiea tsa Kwinbon Fumonisin li ka tšoara limolek'hule tse shebiloeng sebakeng se hloekileng haholo. Hape litšiea tsa Kwinbon li phalla ka potlako, ho bonolo ho sebetsa. Hona joale e sebelisoa ka potlako le ka mokhoa o pharaletseng tšimong ea lijo le lijo-thollo bakeng sa thetso ea mycotoxins.