Elisa Test Kit ea Aflatoxin B1
Litekanyo tse kholo tsa li-aflatoxin li lebisa ho chefo e matla (aflatoxicosis) e ka behang bophelo kotsing, hangata ka tšenyo ea sebete.
Aflatoxin B1 ke aflatoxin e hlahisoang ke Aspergillus flavus le A. parasiticus.Ke carcinogen e matla haholo.Matla ana a carcinogenic a fapana ho ea ka mefuta le tse ling, joalo ka likhoto le litšoene, ho bonahala ho le bonolo ho feta tse ling.Aflatoxin B1 ke tšilafalo e tloaelehileng lijong tse fapaneng tse kenyelletsang matokomane, lijo-thollo tsa cottonseed, poone, le lijo-thollo tse ling;mmoho le dijo tsa diphoofolo.Aflatoxin B1 e nkuwa e le aflatoxin e chefo ka ho fetisisa mme e ameha haholo ho hepatocellular carcinoma (HCC) ho batho.[ho qotsa] Liphoofolo, aflatoxin B1 e boetse e bonts'itsoe e le mutagenic, teratogenic, le ho baka immunosuppression.Mekhoa e mengata ea lisampole le tlhahlobo e kenyelletsang chromatography e tšesaane (TLC), chromatography ea metsi e phahameng haholo (HPLC), mass spectrometry, le enzyme-linked immunosorbent assay (ELISA), har'a tse ling, li sebelisitsoe ho etsa tlhahlobo ea tšilafalo ea aflatoxin B1 lijong. .Ho ea ka Mokhatlo oa Lijo le Temo (FAO), tekanyo ea lefats'e ka bophara e mamelloang ea aflatoxin B1 e tlalehiloe e le 1-20 μg/kg lijong, le 5-50 μg/kg lijong tsa likhomo ka 2003.
Lintlha
1.Elisa Test Kit bakeng sa Aflatoxin B1
2.Katse.KA07202H-96wells
3. Likarolo tsa Kit
● Microtiter plate e koahetsoeng ka antigen, liliba tse 96
● Tharollo e Tloaelehileng ×6bottle(1ml/botlolo)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml………………………………………………………………..…………kepisi e khubelu
● Antibody solution7ml............................................... ...................................................……kepisi e tala
● Substrate A 7ml………………………………………………………………………………………kepisi e tšoeu.
● Substrate B 7ml……………….…………………………………………………….…………kepisi e khubelu
● Stop solution 7ml……….……………………………………………………………..………kepisi e mosehla
● 20×concentrated wash solution 40ml ………………………………………………capparent cap
● 2×tharollo e tebileng ea 50ml……………………………………………………kepisi e putsoa
4.Sensitivity, ho nepahala le ho nepahala
Boikutlo: 0.05ppb
5.Moeli oa ho lemoha
Mohlala oa oli e jeoang ............................................ .................................................. .......................0.1ppb
Peanut................................................. .................................................. .......................0.2ppb
Cereal................................................. .................................................. .....................0.05ppb
Ho nepahala
Mohlala oa oli e jeoang ............................................ .................................................. .................80±15%
Peanut................................................. .................................................. .....................80±15%
Cereal................................................. .................................................. .....................80±15%
Nepahalo:Phapang coefficient ea ELISA kit e ka tlase ho 10%.
6.Cross Rate
Aflatoxin B1 ··················100%
Aflatoxin B2 ·······················81 .3%
Aflatoxin G1 ·······················62%
Aflatoxin G2 ·······················22.3%