Xirmadan ELISA waxaa loogu talagalay in lagu ogaado quinolones iyadoo lagu salaynayo mabda'a immunoassay Enzyme aan tartan toos ahayn. Ceelasha microtiter-ka waxaa lagu dahaadhay antigen-ku-xidhan ee BSA-ga. Quinolones ee muunada ku jira waxay ku tartameysaa antigen-ka lagu dahaadhay saxanka microtitre ee difaaca jirka. Ka dib marka lagu daro konjugate enzyme, substrate chromogenic ayaa la isticmaalaa waxaana calaamada lagu cabbiraa spectrophotometer. Nuugista ayaa si ka duwan u dhiganta fiirsashada quinolones ee muunada.