Shiinaha Immunoaffinity tiirarka ee warshadda ogaanshaha Zearalenone iyo soosaarayaasha

Tiirarka difaaca jirka ee ogaanshaha Zearalenone

Sharaxaad Gaaban:

Tiirarka Kwinbon Zearalenone waxaa loo isticmaalaa iyadoo la isku darayo HPLC, LC-MS, xirmada ELISA. Waxay noqon kartaa tijaabin qiyaas ah AFB1 ee badarka iyo alaabta badarka, maraqa soyka, khalka, maraqa, khamriga, soyga, sabida iyo saliidaha khudradda, quudinta alaabta ceeriin iyo alaabta dhammaatay, iwm.

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Faahfaahinta Alaabta

Tags Product

Tilmaamaha alaabta

Bisadda maya.	KH00304Z
Guryaha	WaayoZearalenonetijaabin
Meesha Asal ahaan	Beijing, Shiinaha
Magaca Summada	Kwinbon
Cabbirka Cutubka	25 imtixaan sanduuq kasta
Tusaalaha Codsiga	Hilibka iyo badarka, maraqa soyka, khalka, waxyaabaha suuga, khamriga, soybeans, seeraha kufsiga iyo saliidda khudradda, quudiyaan alaabta ceeriin iyo alaabta dhammaatay, iwm.
Kaydinta	2-30℃
Nolosha shelf	12 bilood
gaarsiin	Heerkulka qolka

Qalabka & Dib-u-hawlgalayaasha ayaa loo baahan yahay

Qalabka

Reagents

Qalabka

----Homogenizer ---- Isku darka Vortex

----Sample dhalada ----Cabirka dhululubada: 10ml, 100ml

----Waraaqaha shaandhada tayada leh/Centrifuge ---- dheelitirnaanta falanqaynta (inductance: 0.01g)

---- pipette qalin jabiyay: 10ml ----Injector: 20ml

---- Dhalada mugga leh: 250ml ----guluubka pipette-ka caagga ah

----Micropipette: 100-1000ul ---- muraayad muraayad ah 50ml

----Mikrofiber Filters (Whatman, 934-AH, Φ11cm, 1.5um goobada)

Reagents

----Methanol (AR)

----Acetic acid (AR)

----Sodium chloride (NACL,AR)

----Biyo dheecan

Faa'iidooyinka alaabta

Zearalenone (ZEN), oo sidoo kale loo yaqaan RAL iyo F-2 mycotoxin, waa dheef-shiid kiimikaad estrogenic ah oo la soo saaro. Kuleylku waa xasiloon yahay waxaana adduunka oo dhan laga helaa dalagyo badan oo badarka ah, sida galleyda, shaciir, boorash, qamadi, bariis, iyo hadhuudh.

Zearalenone waa sunta asaasiga ah ee ku xirta receptors estrogen, taasoo keenta dhalmo la'aan, ilmo iska soo ridid ama dhibaatooyinka kale ee taranka, gaar ahaan doofaarka.

Wikipedia waxay ku talinaysaa hababka ogaanshaha soo socda;

chromatography lakabka khafiifka ah,
Baaritaanka difaaca jirka ee Enzyme-ku xiran
fluorescence tiirarka difaaca jirka
Tiirka difaaca jirka chromatography dareeraha waxqabadka sare

Tiirarka Inmmunoaffinity Kwinbon ayaa iska leh habka saddexaad, waxay u isticmaashaa chromatography dareeraha ah ee kala-soocidda, nadiifinta ama falanqaynta gaarka ah ee Zearalenone. Caadi ahaan tiirarka Kwinbon waxaa lagu daraa HPLC.

Falanqaynta tirada HPLC ee sunta fangasku waa farsamo ogaansho bislaaday. chromatography wejiga hore iyo gadaalba labadaba waa lagu dabaqi karaa. Marxaladda rogaal celiska ah ee HPLC waa mid dhaqaale, fududahay in lagu shaqeeyo, oo waxay leedahay sunta dareeraha oo yar. Inta badan sunta waxay ku milmaan wejiyada wareega wareega ee polar ka dibna ay kala soocaan tiirarka chromatography ee aan cirridka ahayn, iyaga oo buuxinaya baahida degdegga ah ee sunta fangas ee badan ee saamiga caanaha. Qalabka UPLC ee isku dhafan ayaa si tartiib tartiib ah loo dabaqayaa, oo leh cutubyo cadaadis sare leh iyo cabbir yar iyo cabbirka qayb ka mid ah tiirarka chromatography, kaas oo soo gaabin kara muunada wakhtiga socodsiinta, hagaajinta waxtarka kala-soocidda chromatographic, oo gaaro dareen sare.

Si gaar ah u sarreeya, tiirarka Kwinbon Zearalenone waxay qaban karaan molecules bartilmaameedka xaalad aad u nadiif ah. Sidoo kale tiirarka Kwinbon waxay u socdaan si degdeg ah, oo sahlan in la shaqeeyo. Hadda waxa si degdeg ah oo ballaadhan loogu isticmaalayaa quudinta iyo beerta hadhuudhka si loo khiyaamo mycotoxins.