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Qalabka Tijaabada ee Elisa ee Ochratoxin A Sawirka Sifaysan
  • Qalabka Imtixaanka Elisa ee Ochratoxin A
  • Qalabka Imtixaanka Elisa ee Ochratoxin A

Qalabka Imtixaanka Elisa ee Ochratoxin A

Sharaxaad Gaaban:

Qalabkan waxa loo isticmaali karaa qiyaas ahaan iyo falanqaynta tayada leh ee ochratoxin A ee quudinta.Waa badeeco cusub oo loogu talagalay ogaanshaha haraaga daroogada ee ku saleysan tignoolajiyada ELISA, kaas oo kaliya ku kacaya 30min hawlgal kasta oo si weyn u yareeyn kara khaladaadka hawlgalka iyo xoojinta shaqada.Xirmadan waxay ku salaysan tahay tignoolajiyada ELISA ee aan tooska ahayn ee tartanka.Ceelasha microtiter-ka waxaa lagu dahaadhay antigen-ka isku-xidha.Ochratoxin A oo muunad ku jirta waxa ay kula tartameysaa antigen-ka lagu dahaadhay saxanka microtiter si loogu daro antibody .Ka dib marka lagu daro konjugate enzyme, substrate TMB ayaa loo isticmaalaa si loo muujiyo midabka.Nuugista muunada waxay si xun ula xiriirtaa haraaga o chratoxin A ee ku jira, ka dib marka la barbardhigo Curve Standard, oo lagu dhufto qodobbada milanka, Ochratoxin A tirada muunada waa la xisaabin karaa.


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Faahfaahinta Alaabta

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Ochratoxins waa koox mycotoxins ah oo ay soo saaraan qaar ka mid ah noocyada Aspergillus (badanaa A).Ochratoxin A waxaa loo yaqaanaa inay ku dhacdo badeecadaha sida badarka, qaxwaha, khudradda la qalajiyey iyo khamriga cas.Waxaa lagu tiriyaa kansarka bani'aadamka oo ay leedahay dano gaar ah maadaama ay ku urursan karto hilibka xoolaha.Markaa hilibka iyo hilibka hilibka ayaa lagu sumayn karaa suntan.Soo-gaadhista ochratoxins iyada oo loo marayo cuntada waxay yeelan kartaa sun daran kelyaha naasleyda, waxayna noqon kartaa kansar.

Faahfaahin

1. Qalabka Imtixaanka Elisa ee Ochratoxin A

2. Bisad.KA07301H-96 Ceelasha

3. Qaybaha Kit
● Saxan yar oo leh 96 ceel oo lagu dahaadhay antigen
● xalalka caadiga ah (6 dhalo: 1ml/dhalo)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
● Enzyme conjugate 7ml………………………………………………………………………………………………………
Xalka difaaca jirka 10ml………………………………………………………………………………………….…. fur cagaaran
Xalka substrate A 7ml…………………………………………………………………………………………………………………………………
Xalka substrate B 7ml…………………………………………………………………………………………………………
● Jooji xal 7ml …………………………………………………………………………………………………………
20× Xalka dhaqida ee xooga leh 40ml………………………………………………………………………

4. Dareenka, saxnaanta iyo saxnaanta
Dareenka Imtixaanka: 0.4ppb
Xadka ogaanshaha
Quudinta …………………………………………………………………………………………………………… 5ppb
Saxnaanta
Quudinta………………………………………………………………………………………………………………………………………
Saxnaanta:Kala duwanaanshaha xirmada ELISA waa in ka yar 10%.

5. Heerka isdhaafsiga
Ochratoxin A………………………………………………………………………………………………………………..100%

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