Qalabka Imtixaanka Elisa ee Ochratoxin A
Ku saabsan
Qalabkan waxa loo isticmaali karaa qiyaas ahaan iyo falanqaynta tayada leh ee ochratoxin A ee quudinta.Waa badeeco cusub oo loogu talagalay ogaanshaha haraaga daroogada ee ku saleysan tignoolajiyada ELISA, kaas oo kaliya ku kacaya 30min hawlgal kasta oo si weyn u yareeyn kara khaladaadka hawlgalka iyo xoojinta shaqada.Xirmadan waxay ku salaysan tahay tignoolajiyada ELISA ee aan tooska ahayn ee tartanka.Ceelasha microtiter-ka waxaa lagu dahaadhay antigen-ka isku-xidha.Ochratoxin A oo muunad ku jirta waxa ay kula tartameysaa antigen-ka lagu dahaadhay saxanka microtiter si loogu daro antibody .Ka dib marka lagu daro konjugate enzyme, substrate TMB ayaa loo isticmaalaa si loo muujiyo midabka.Nuugista muunada waxay si xun ula xiriirtaa haraaga o chratoxin A ee ku jira, ka dib marka la barbar dhigo Curve Standard, oo lagu dhufto qodobbada milanka,Ochratoxin Tiro ka mid ah muunada waa la xisaabin karaa.
Qaybaha Kit
• saxan Microtiter leh 96 ceel oo lagu dahaadhay antigen
•Sxalalka tandard (6 dhalo: 1ml/dhalo)
0ppb, 0.4ppb, 0.8ppb, 1.6ppb, 3.2ppb, 6.4ppb
• Enzymeisku xidhid7ml …………………………………………………………………..………..…..koofiyad cas
• Xalka difaaca jirka10ml …………………………………………………………………………...….…koofi cagaaran
•Substrate smidabka A 7ml………………………………………………………………………………………………………………………………
•SubstrateXalka B 7ml………………………………………………………………………………..………………… koofiyad cas
Jooji xal 7ml ………………………………………………………………………………….………………………… Koofi huruud ah
• 20× Xalka dhaqidda ee xoogga leh 40ml………...………………………………….…...… dabool hufan
Dareenka, saxnaanta iyo saxnaanta
Dareenka Imtixaanka: 0.4ppb
Xadka ogaanshaha
Quudinta……………………………………………………….………………………………………… 5ppb
Saxnaanta
Quudinta……………………………………………………………….………….90±20%
Saxnaanta
Kala duwanaanshaha xirmada ELISA waa in ka yar 10%.
Heerka isdhaafsiga
Ochratoxin A………………………………………………………..…………………..100%