Qalabka Imtixaanka Elisa ee AOZ
Nitrofurans waa antibiyootig ballaadhan oo synthetic ah, kuwaas oo inta badan lagu isticmaalo wax soo saarka xayawaanka si ay u helaan bakteeriyada ugu fiican iyo daawooyinka farmashiyaha.
Waxa kale oo loo isticmaali jiray kobcinta korriinka doofaarka, digaaga iyo wax soo saarka biyaha.Daraasado muddo dheer lagu sameeyay xayawaanka shaybaadhka ayaa muujiyay in dawooyinka waalidka iyo dheef-shiid kiimikaadkoodu ay muujiyeen astaamaha kansarka iyo mutagenic.Daawooyinka nitrofuran furaltadone, nitrofurantoin iyo nitrofurazone ayaa laga mamnuucay isticmaalka wax soo saarka xoolaha ee EU 1993, isticmaalka furazolidone waa la mamnuucay 1995.
Faahfaahin
1.Elisa Qalabka Imtixaanka ee AOZ
2.Bisad.A008-96 Ceelasha
3.Qalabka ka kooban
● saxan Microtiter ah oo lagu dahaadhay antigen, 96 ceel
● xalalka caadiga ah (6 dhalo, 1ml/dhalo)
0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb
● Xakamaynta caadiga ah ee daada: (1ml/ dhalo)................................................ .......100ppb
● Enzyme conjugate conjugate 1.5ml.......................................... ...... koofiyad cas
● Xalka difaaca jirka waxa uu uruursaday 0.8ml………………………………………………
● Substrate A 7ml………………………………………………. ......................... Koofi cad
● Substrate B7ml……………………………………………………………………… .........................
● Jooji xal 7ml………………………………………………………………
20× Xalka dhaqidda ee xoogga leh 40ml ………………………………………. dabool hufan
2× 60ml saarista lasaaray
2-Nitrobenzaldehyde 15.1mg………………………………………………………
4.Dareenka, saxnaanta iyo saxnaanta
Dareenka: 0.025ppb
Xadka ogaanshaha ………………………………………………………………………….1ppb
Saxnimada:
Unugyada xoolaha (muruqa iyo beerka)……………………………………… 75± 15%
Malabka……………………………………………………………………………………………….90±20%
Ukun………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………
Caano………………………………………………………………………………………………
Saxnimada: CV ee xirmada ELISA waa in ka yar 10%.
5.Cross rate
Furazolidone metabolite (AOZ) …………………………………………………………………………………..
Furaltadone metabolite (AMOZ)………………………………………………………
Nitrofurantoin metabolite (AHD) …………………………………………………………………………………………………….
Nitrofurazone metabolite (SEM) …………………………………………………………………………………….
Furazolidone …………………………………………………………………………………………………………………
Feraltadone ............................................... ...
Nitrofurantoin……………………………………………………………………………………….. <1%
Nitrofurazone …………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………………….