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Qalabka Imtixaanka Elisa ee Sawirka AMOZ
  • Qalabka Imtixaanka Elisa ee AMOZ
  • Qalabka Imtixaanka Elisa ee AMOZ
  • Qalabka Imtixaanka Elisa ee AMOZ
  • Qalabka Imtixaanka Elisa ee AMOZ

Qalabka Imtixaanka Elisa ee AMOZ

Sharaxaad Gaaban:

Daawooyinka nitrofuran furaltadone, nitrofurantoin iyo nitrofurazone ayaa laga mamnuucay isticmaalka wax soo saarka xayawaanka cuntada ee EU 1993, isticmaalka furazolidone waa la mamnuucay 1995. Falanqaynta daawooyinka nitrofuran ayaa loo baahan yahay in lagu saleeyo ogaanshaha dheef-shiid kiimikaadka ku xiran unugyada Daawooyinka waalidka ee nitrofuran, maadaama daawooyinka waalidku ay si dhakhso ah u dheefshiidaan, iyo unugyada nitrofuran metabolites ee ku xiran waxay sii hayn doonaan waqti dheer, sidaas darteed dheef-shiid kiimikaadka ayaa loo isticmaalaa bartilmaameedka lagu ogaanayo xadgudubka nitrofurans.Furazolidone metabolite (AMOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) iyo Nitrofurazone metabolite (SEM).

BisadKA00205H-96 Ceelasha


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Ku saabsan

Qalabkan waxa loo isticmaali karaa qiyaas ahaan iyo falanqayn tayo leh oo ku saabsan hadhaaga AMOZ ee alaabta biyaha (kalluunka iyo shrimp), iwm

Xirmadan waxaa loogu talagalay in lagu ogaado AMOZ iyadoo lagu salaynayo mabda'a tallaalka difaaca jirka ee aan tooska ahayn.Ceelasha microtiter-ka waxa lagu dahaadhay qabashada BSA oo ku xidhan

antigen.AMOZ muunada waxay la tartameysaa antigen-ka lagu dahaadhay saxanka microtiter ee difaaca jirka lagu daray.Ka dib marka lagu daro enzyme conjugate , substrate chromogenic ayaa la isticmaalaa waxaana calaamada lagu cabbiraa spectrophotometer.Nuugista ayaa si ka duwan u dhiganta AM OZ-fiirinta saamiga.

Qaybaha Kit

Saxan microtiter ah oo leh 96 ceel oo lagu dahaadhay antigen

Xalalka caadiga ah (6 dhalo)

0ppb, 0.05ppb,0.15ppb,0.45ppb,1.35ppb,4.05ppb

Xalka caadiga ah ee spiking: (1ml/ dhalo) ………………………………………………………………….100ppb

Enzyme conjugate 1ml…………………………………………………………………………………

Xalka difaaca jirka 7ml ……………………………………………………………………………. fur cagaaran

xal A 7ml……………………………………………………………………….………………… koofiyad cad

xal B 7ml……………………………………………………………………………….……………. koofiyad cas

Xalka joojinta 7ml……………………………………………………………….………… Koofi huruud ah

· 20× xal dhaqid oo xoog leh 40ml………………………………………………………

2× Xalka soo saarista xooga leh 50ml………………………………………………………

2-Nitrobenzaldehyde 15.1mg………………………………………………………………………

Dareenka, saxnaanta iyo saxnaanta

Dareenka: 0.05ppb

Xadka ogaanshaha

Alaabooyinka biyaha (kalluunka iyo shrimp) ………………………………… 0.1ppb

Saxnaanta

Alaabooyinka biyaha (kalluunka iyo shrimp) ………………………………… 95 ± 25%

Saxnaanta:CV ee xirmada ELISA waa in ka yar 10%.

Heerka isdhaafsiga

Furaltadone metabolite (AMOZ) ………………………………………….…………100%

Furazolidone metabolite (AMOZ) …………………………………………………...………………………….<0.1%

Nitrofurantoin metabolite (AHD)………………………………………………..………………… <0.1%

Nitrofurazone metabolite (SEM) ………………………………………………….… <0.1%

Furaltadone ………………………………………………………………….…….11.1%

Furazolidone……………………………………………………….………… <0.1%

Nitrofurantoin……………………………………………………………………………………………….. <1%

Nitrofurazone ………………………………………………………….………………………… <1%

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