Qalabka Tijaabada ee Elisa ee Aflatoxin B1
Ku saabsan
Xirmadan waxa loo isticmaali karaa in lagu qiimeeyo tayada iyo falanqaynta aflatoxin B1 ee saliidda la cuni karo, lawska, badarka badarka, maraqa soyka, khalka iyo quudinta (quudinta cayriin, agabka isku qasan iyo agabka la isku daray). falanqaynta qalabka.
Alaabtani waxay ku salaysan tahay tartanka tooska ah ee ELISA, kaas oo degdeg ah, sax ah oo xasaasi ah marka la barbar dhigo falanqaynta qalabka caadiga ah.Waxay u baahan tahay kaliya 45min hal hawlgal, kaas oo si weyn u yarayn kara qaladka hawlgalka iyo xoojinta shaqada.
Qaybaha kitka
• Microtiter platededeshed leh antigen, 96 ceel
Xalka caadiga ah ×6 dhalo (1ml/dhalo)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
Enzyme conjugate 7ml………………………………………………………………………………
• Xalka difaaca jirka7ml.............................................................. ...........………… Koofi cagaaran
Substrate A 7ml……………………………………………………………………….…………………. Koofi cad
Substrate B 7ml……………………………………………………………………………….………… koofiyad cas
Jooji xalka 7ml……………………………………………………………………………….………… koofiyad huruud ah
20× Xalka dhaqidda ee xoogga leh 40ml …………………………………………………………
2× Xalka soo saarista xooga leh 50ml………………………………………………………………………
Dareenka, saxnaanta iyo saxnaanta
Dareenka0.05ppb
Xadka ogaanshaha
Saliida la cuni karo.................................. .................................................................0.1ppb
Lawska................................................. ................................................... ......................0.2ppb
badarka................................................ ................................................... ......................0.05ppb
Saxnaanta
Saliida la cuni karo.................................. ...................................................................80± 15%
Lawska................................................. ................................................... ......................80± 15%
badarka................................................ ................................................... ......................80± 15%
Saxnaanta
Kala duwanaanshaha xirmada ELISA waa in ka yar 10%.
Heerka isdhaafsiga
Aflatoxin B1· 100%
Aflatoxin B2··················································81 .3%
Aflatoxin G1····························································································.%
Aflatoxin G2·····························································22.3%