Pakati pekuwedzera kwakasimba kwenyaya dzekuchengetedza chikafu, rudzi rutsva rwebvunzo kitero yakavakirwa paneEnzyme-linked immunosorort assay (Elisa)zvishoma nezvishoma kuva chishandiso chakakosha mumunda wekuedzwa kwekuchengetedza kwekudya. Izvo hazvingogovanisi zvakanyatsojeka uye kushanda zvakanaka zvekutarisa kwekudya kwekudya asiwo vanovaka mutsara wekudzivirira wekudzivirira kwekuchengetedza kwevatengi.
Iyo nheyo yeye Elisa bvunzo kit iri kushandisa iyo chaiyo yekusunga yekusunga pakati peantifu uye antibody yekufungidzira zvinhu zvechikafu mune zvekudya kuburikidza neEnzyme-carlyzed substrate budiriro. Maitiro ayo ekushanda anenge ari nyore uye ane ruzivo rwakanyanya uye nekunzwa, kuita kuti kuonekera kwakaringana uye kuyerwa kwezvinhu zvinokuvadza pakudya, zvakadai saAfrica, Ochratoxin a, uyeT-2 Toxins.
Panyaya yeanotaura maitiro ekushanda, iyo Elisa test kit kazhinji inosanganisira iyo inotevera matanho:
1. Kuenzanisa: Chekutanga, sampuli yekuyedzwa kuti iongororwe inofanirwa kuongororwa nenzira yakakodzera, sekutsamwa nekucheneswa, kuwana mhinduro yemuenzaniso iyo inogona kushandiswa pakuonekwa.
2..
3. Incubation: iyo Elisa ndiro yakawedzerwa samples yakawedzerwa pane tembiricha yakakodzera kwenguva yenguva yekubvumira kubhururuka kwakazara pakati pekutorwa uye antibodies.
4
5.Substrate nekuvandudza mavara: Substrate Solution inowedzerwa kune imwe neimwe tsime, uye enzyme pane enzyme-anonzi Anti Antibody carlyzes yekunyora ruvara, ichigadzira chigadzirwa chemavara.
6. Kuyerwa Zviri mukati meiyo chinhu chinofanira kuedzwa chinobva chave chakaverengerwa zvichienderana neyakajairwa curve.
Pane nyaya dzakawanda dzekushandisa dzeElisa Bvunzo dzeElisa mukuongororwa kwekuchengetedza kwekudya. Semuenzaniso, panguva yekuwedzera kwekudya kwekuchengetedza uye sampling kuongorora, zviremera zvekurongedza zveMusika zvakashandisa iyo Elisa Test kit yekukurumidza uye nekunyatsoongorora mazinga akawandisa e Aflatoxin b1 muPeanut mafuta anogadzirwa neayo emafuta. Matanho akakodzera akakodzera akakurumidza kutorwa, zvinobudirira kudzivirira chinhu chinokuvadza kubva kunze kwekuparadza vatengi.
Uyezve, nekuda kwekurererwa kwaro kwekushanda, kunyatsoita, uye kuvimbika, iyo Elisa test kit iri kushandiswa zvakanyanya mukuchengetedzeka kwezvekudya zvakasiyana-siyana zvigadzirwa, zvigadzirwa nyama, uye zvigadzirwa zvemukaka. Izvo hazvingopfupise zvakanyanya nguva yekuona uye inovandudza kushanda zvakanaka asi zvakare inopa kune vane simba tekinoroji kutsigirwa kwevakuru vemauto kuti vasimbise kutarisirwa kwemusika wekudya.
Nekuenderera mberi kwekufambira mberi kwehunyanzvi uye nekuziva kuwedzera kuchengetedzeka kwekudya pakati pevanhu, Elisa bvunzo kits kuchaita basa rinowedzera kukosha mumunda wekuedzwa kwekuchengetedza chikafu. Mune ramangwana, tinotarisira kuwedzera kwehunyanzvi hwekugadzira tekinoroji, kusimudzira kusimudzira kusimba kwesisitiri yekuchengetedza kwekudya uye kupa chivimbo chakasimba chekuchengetedza kwekuchengetedza kwevatengi.
Kutumira Nguva: Dec-12-2024