Pakati pekuwedzera kuoma kwenyaya dzekuchengetedza chikafu, rudzi rutsva rwetest kit yakavakirwa paneEnzyme-Yakabatanidzwa Immunosorbent Assay (ELISA)zvishoma nezvishoma iri kuva chishandiso chakakosha mumunda wekuongorora kuchengetedza kwekudya. Iyo haingopi chete yakanyatsojeka uye inoshanda nzira dzekutarisa kunaka kwechikafu asiwo inovaka tambo yakasimba yekudzivirira kuchengetedza chikafu chevatengi.
Iyo musimboti weELISA test kit iri mukushandisa chaiyo inosunga maitiro pakati peantigen neantibody kuona muhuwandu zviri mukati mezvinhu zvinonangwa muchikafu kuburikidza ne enzyme-catalyzed substrate color development. Maitiro ayo ekushanda ari nyore uye ane hunyanzvi hwepamusoro uye kunzwisiswa, achigonesa kuzivikanwa uye kuyerwa kwezvinhu zvinokuvadza muchikafu, senge aflatoxin, ochratoxin A, uye.T-2 chepfu.
Panyaya yemaitiro chaiwo ekushanda, iyo ELISA test kit inowanzo sanganisira anotevera matanho:
1. Kugadzirira kwemuenzaniso: Kutanga, chikafu chekudya chinofanirwa kuongororwa chinoda kugadziriswa zvakakodzera, zvakadai sekubvisa uye kucheneswa, kuwana mhinduro yemuenzaniso inogona kushandiswa kuonekwa.
2. Sample yekuwedzera: Mhinduro yemuenzaniso yakagadziriswa inowedzerwa kumatsime akasarudzwa muELISA ndiro, netsime rimwe nerimwe rinoenderana nechinhu chinofanira kuongororwa.
3. Incubation: Iyo ELISA ndiro ine masamples akawedzerwa inoiswa patembiricha yakakodzera kwenguva yakati rebei kubvumira kusungwa kuzere pakati pemaantigen nemasoja ekudzivirira chirwere.
4. Kushamba: Mushure mokuputira, mushonga wekugeza unoshandiswa kubvisa maantigen asingasviki kana ma antibodies, kuderedza kupindira kwekusungirirwa kusingatarisirwi.
5.Substrate yekuwedzera uye kukura kwemavara: Substrate mhinduro inowedzerwa kune rimwe nerimwe tsime, uye iyo enzyme pane enzyme-yakanyorwa antibody inokonzeresa iyo substrate kugadzira ruvara, ichigadzira chigadzirwa chine ruvara.
6. Chiyero: Kunyura kukosha kwechigadzirwa chemavara mutsime rega rega chinoyerwa uchishandisa zviridzwa zvakaita semuverengi weELISA. Zviri mukati mechinhu chichayedzwa chinobva chaverengerwa pachishandiswa curve yemazuva ese.
Kune akawanda ekushandisa makesi eELISA bvunzo kits mukuyedzwa kwekuchengetedza chikafu. Semuyenzaniso, panguva yekutarisa kuchengetedza chikafu uye ongororo yesampling, zviremera zvinodzora misika zvakashandisa ELISA test kit kuona nekukurumidza uye nemazvo mazinga eaflatoxin B1 mumafuta enzungu anogadzirwa nechigayo chemafuta. Matanho akakodzera echirango akatorwa nekukasika, achidzivirira zvinokuvadza zvinhu kubva pakuisa mungozi vatengi.
Zvakare, nekuda kwekureruka kwekushanda, chokwadi, uye kuvimbika, iyo ELISA test kit inoshandiswa zvakanyanya mukuyedzwa kwekuchengetedza kwezvikafu zvakasiyana senge zvigadzirwa zvemumvura, zvigadzirwa zvenyama, uye zvigadzirwa zvemukaka. Iyo haingopfupiki zvakanyanya nguva yekuona uye inonatsiridza kushanda zvakanaka asiwo inopa yakasimba tekinoroji tsigiro yezviremera zvinodzora kusimbisa kutarisa kwemusika wekudya.
Nekuenderera mberi kwekufambira mberi kwehunyanzvi uye kuwedzera kuziva kwekuchengetedza chikafu pakati pevanhu, ELISA test kits ichaita basa rinowedzera kukosha mumunda wekuyedzwa kwekuchengetedza chikafu. Mune ramangwana, tinotarisira kuenderera mberi kwekubuda kwehunyanzvi hwekuvandudza tekinoroji, pamwe chete kusimudzira kusimudzira kwakasimba kweindasitiri yezvekuchengetedza chikafu uye nekupa vimbiso yakasimba yekuchengetedza chikafu chevatengi.
Nguva yekutumira: Zvita-12-2024