Elisa Test Kit yeCAP
Chloramphenicol inorwisa mabhakitiriya inobudirira, inowanzoshandiswa mukurapa zvirwere zvakasiyana-siyana zvinotapukira zvemhuka, uye ine inhibitory mhedzisiro pane akasiyana mabhakitiriya epathogenic.Dambudziko rakakomba nemasara echloramphenicol.Chloramphenicol ine chepfu yakakomba uye mhedzisiro, iyo inogona kutadzisa hematopoietic basa remapfupa emunhu, zvichikonzera munhu aplastic anemia, granular leukocytosis, neonatal, premature grey syndrome uye zvimwe zvirwere, uye kuderera kwehuwandu hwemasara emishonga kunogona kukonzera chirwere.Naizvozvo, masara echloramphenicol muchikafu chemhuka anoisa njodzi huru kuhutano hwevanhu.Naizvozvo, yakarambidzwa kana kushandiswa zvakaganhurirwa muEU neUS.
Kwinbon iyi kit chigadzirwa chitsva chakavakirwa paELISA, inokurumidza (50min chete mukushanda kumwe), iri nyore, yakarurama uye inonzwisisika zvichienzaniswa neyakajairwa ongororo yezviridzwa, uye saka inogona kuderedza zvakanyanya kukanganisa kwekushanda uye kusimba kwebasa.
Cross-reactions
Chloramphenicol ……………………………………..……100%
Chloramphenicol palmitate ……………………………… <0.1%
Thiamphenicol………………………………………………..<0.1%
Florfenicol………………………………………………… <0.1%
Cetofenicol…………………………………………..…<0.1%
Kit Zvikamu
Microtiter ndiro yakavharwa neantigen, 96wells
Yakajairika mhinduro (6×1ml/bhodhoro)
0ppb,0.025ppb,0.075ppb,0.3ppb,1.2ppb,4.8ppb
Spiking standard solution: (1ml/bhodhoro) …….…100ppb
Concentrated enzyme conjugate 1ml….…..……………..….…..….…..….……….. transparent cap
Enzyme conjugate diluent 10ml….…..….…………………..…..….…..….…..…..transparent cap
Solution A 7ml ………………………………………… ..............….………….kapu chena
Solution B 7ml…………………………………………………… .........…….……….. chivharo chitsvuku
Stop solution 7ml ……………………………………… .............….…….…..yellow cap
20×Concentrated wash solution 40ml…………………………………………….….transparent cap
2 × Yakanyanya kudhirowa mhinduro 50ml .......................................... ...........kapu yeblue
Results
1 Percentage absorbance
Izvo zvinorehwa maitiro ezviyero zvekutora zvakawanikwa kune zviyero uye sampuli dzakakamurwa nekunyura kukosha kweyero yekutanga (zero standard) uye inowedzerwa ne100%.Iyo zero chiyero inoitwa yakaenzana ne100% uye iyo yekutora tsika inotorwa muzvikamu.
B --absorbance standard (kana sampuli)
B0 —-absorbance zero standard
2 Standard Curve
Kudhirowa yakajairwa curve: tora kukosha kwekunyudza kwemazinga se y-axis, semi logarithmic yekusangana kweCAP zviyero mhinduro (ppb) se x-axis.
Iko CAP kusungirirwa kwemuenzaniso wega wega (ppb), iyo inogona kuverengwa kubva pachiyero chekugadzirisa, inowedzerwa neinowirirana Dilution factor yemuenzaniso wega wega unoteverwa, uye iyo chaiyo yekuenzanisa yemuenzaniso inowanikwa.
Tapota cherechedzai:
Kuti uwane data yekuongorora yeELISA kits, yakakosha software yakagadziridzwa, iyo inogona kurairwa pakukumbira.
