chigadzirwa

About

Iyi kiti inogona kushandiswa muhuwandu uye hwemhando yekuongorora kweAOZ zvakasara mumatishu emhuka (huku, mombe, nguruve, nezvimwewo), mukaka, uchi nemazai.

Kuongororwa kwemishonga ye nitrofuran yakasara inoda kuenderana nekuonekwa kwehutachiona hwakasungwa metabolites yemishonga yevabereki nitrofuran, iyo inosanganisira Furazolidone metabolite (AOZ), Furaltadone metabolite (AMOZ), Nitrofurantoin metabolite (AHD) uye Nitrofurazone metabolite (SEM).

Kuenzaniswa nenzira dzechromatographic, kititi chedu chinoratidza zvakakura zvakanaka maererano nekunzwa, muganho wekuona, tekinoroji michina uye nguva inodiwa.

Kit Zvikamu

• Microtiter plate yakavharwa neantigen, 96 matsime

• Mishonga yemhando (6 mabhodhoro, 1ml/bhodhoro)

0ppb,0.025ppb,0.075ppb,0.225ppb,0.675ppb,2.025ppb

• Spiking standard control : (1ml/bhodhoro)..............................................….100ppb

• Enzyme conjugate concentrate 1.5ml.............................................….….kapu dzvuku

• Mushonga wemasoja ekudzivirira chirwere akaiswa 0.8ml………………………………......kapu yegirinhi

• Substrate A 7ml…………………...........................................…………..kapu chena

• Substrate B7ml………………………..................................................... chivharo chitsvuku

• Stop solution 7ml………………………………………………….………yellow cap

• 20×concentrated wash solution 40ml …………………………….

• 2×concentrated extraction solution 60ml……………………..…………….blue cap

• 2-Nitrobenzaldehyde 15.1mg……………………………………………………

Sensitivity, kururama uye nemazvo

Sensitivity: 0.025ppb

Detection limit……………..…………………………………

Kururama:

Zvinyama zvemhuka (tsandanyama nechiropa)……….………….75±15%

Uchi ……………………………………………………..90±20%

Zai……………………………………………………..…90±20%

Mukaka……………………………………………………..…90±10%

Precision:CV ye ELISA kit iri pasi pe10%.

Cross Rate

Furazolidone metabolite (AOZ)……………………………………………..100%

Furaltadone metabolite (AMOZ)…………………………………………<0.1%

Nitrofurantoin metabolite (AHD)……………………………………… <0.1%

Nitrofurazone metabolite (SEM)…………………………………………<0.1%

Furazolidone………………………………………………………….…..…16.3%

Furaltadone…………………………………………………………….…<1%

Nitrofurantoin………………………………………………………….…<1%

Nitrofurazone……………………………………………………………..…<1%