chigadzirwa

Mahombe madosi eaflatoxins anotungamira kune acute chepfu (aflatoxicosis) inogona kutyisidzira hupenyu, kazhinji kuburikidza nekukuvadza kuchiropa.
Aflatoxin B1 iaflatoxin inogadzirwa neAspergillus flavus uye A. parasiticus.Iyo ine simba zvikuru carcinogen.Iyi carcinogenic potency inosiyana pakati pezvipenyu nedzimwe, senge makonzo netsoko, zvinoita kunge zviri nyore kupfuura dzimwe.Aflatoxin B1 inowanzosvibiswa mune zvakasiyana-siyana zvekudya zvinosanganisira nzungu, cottonseed meal, chibage, nezvimwe zviyo;pamwe chete nezvokudya zvezvipfuwo.Aflatoxin B1 inoonekwa seaflatoxin ine muchetura uye inonyanya kukonzeresa muhepatocellular carcinoma (HCC) muvanhu.Nzira dzinoverengeka dzesampling uye dzekuongorora dzinosanganisira dzakatetepa-layer chromatography (TLC), yakakwira-performance liquid chromatography (HPLC), mass spectrometry, uye enzyme-yakabatana immunosorbent assay (ELISA), pakati pevamwe, yakashandiswa kuyedza kusvibiswa kweaflatoxin B1 muchikafu. .Sekureva kweFood and Agriculture Organisation (FAO), huwandu hwepasi rose hunoshivirira huwandu hweaflatoxin B1 hwakanzi hwaive pakati pe1-20 μg/kg muchikafu, uye 5–50 μg/kg mukudya kwemombe muna 2003.

Details

1.Elisa Test Kit yeAflatoxin B1

2.Katsi.KA07202H-96wells

3. Zvikamu zvekiti
● Microtiter plateprecoated with antigen, 96 matsime
● Standard Solution ×6bhodhoro(1ml/bhodhoro)
0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb
● Enzyme conjugate 7ml……………………………………………………………..…………kapu tsvuku
● Antibody solution7ml............................................. ..................................................... green cap
● Substrate A 7ml……………………………………………………………………………………chivharo chichena
● Substrate B 7ml……………….…………………………………………………….…………kapu tsvuku
● Stop solution 7ml……….…….……………………………………………………..………yellow cap
● 20×concentrated wash solution 40ml ………………………………………………
● 2×concentrated extraction solution 50ml…………………………………………………

4.Sensitivity, yakarurama uye yakarurama
Sensitivity: 0.05ppb

5.Detection muganhu
Sampuli yemafuta anodyiwa .......................................... .................................................. .......................0.1ppb
Nzungu................................................. .................................................. .......................0.2ppb
Zviyo................................................. .................................................. .....................0.05ppb
Kururama
Sampuli yemafuta anodyiwa .......................................... .................................................. ..................80±15%
Nzungu................................................. .................................................. .....................80±15%
Zviyo................................................. .................................................. .....................80±15%
Precision:Kusiyanisa coefficient ye ELISA kit iri pasi pe10%.

6.Cross Rate
Aflatoxin B1··················100%
Aflatoxin B2·······················81 .3%
Aflatoxin G1·······················62%
Aflatoxin G2 ·······················22.3%