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Elisa Test Kit yeAflatoxin B1 Inoratidzwa Mufananidzo
  • Elisa Test Kit yeAflatoxin B1
  • Elisa Test Kit yeAflatoxin B1
  • Elisa Test Kit yeAflatoxin B1

Elisa Test Kit yeAflatoxin B1

Tsanangudzo Pfupi:

Mahombe madosi eaflatoxins anotungamira kune acute chepfu (aflatoxicosis) inogona kutyisidzira hupenyu, kazhinji kuburikidza nekukuvadza kuchiropa.

Aflatoxin B1 iaflatoxin inogadzirwa neAspergillus flavus uye A. parasiticus.Iyo ine simba zvikuru carcinogen.Iyi carcinogenic potency inosiyana pakati pezvipenyu nedzimwe, senge makonzo netsoko, zvinoita kunge zviri nyore kupfuura dzimwe.Aflatoxin B1 inowanzosvibiswa mune zvakasiyana-siyana zvekudya zvinosanganisira nzungu, cottonseed meal, chibage, nezvimwe zviyo;pamwe chete nezvokudya zvezvipfuwo.Aflatoxin B1 inoonekwa seaflatoxin ine muchetura uye inonyanya kukonzeresa muhepatocellular carcinoma (HCC) muvanhu.Nzira dzinoverengeka dzesampling uye dzekuongorora dzinosanganisira dzakatetepa-layer chromatography (TLC), yakakwira-performance liquid chromatography (HPLC), mass spectrometry, uye enzyme-yakabatana immunosorbent assay (ELISA), pakati pevamwe, yakashandiswa kuyedza kusvibiswa kweaflatoxin B1 muchikafu. .Sekureva kweFood and Agriculture Organisation (FAO), huwandu hwepasi rose hunoshivirira huwandu hweaflatoxin B1 hwakanzi hwaive pakati pe1-20 μg/kg muchikafu, uye 5–50 μg/kg mukudya kwemombe muna 2003.


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Iyi kit inogona kushandiswa kuongororwa kwemhando uye kuwanda kweaflatoxin B1 mumafuta anodyika, nzungu, zviyo, soy muto, vhiniga uye chikafu (chikafu chekudya, zvakasanganiswa batch zvinhu uye zvakasanganiswa zvinhu). ongororo yezvishandiso.

Ichi chigadzirwa chakavakirwa pane isina kunanga inokwikwidza ELISA, iyo inokurumidza, yakarurama uye inonzwisisika kana ichienzaniswa neyakajairwa chimbo chekuongorora.Inongoda 45min chete mukushanda kumwe, izvo zvinogona kuderedza zvakanyanya kukanganisa kwekushanda uye kushanda nesimba.

Kit zvikamu

• Microtiter plateprecoated ne antigen, 96 matsime

• Standard Solution ×6bhodhoro(1ml/bhodhoro)

0ppb, 0.02ppb, 0.06ppb, 0.18ppb, 0.54ppb, 1.62ppb

• Enzyme conjugate 7ml………………………………………………………………..…………kapu tsvuku

• Antibody solution7ml................................................................. .................………green cap

• Substrate A 7ml……………………………………………………………….………………kepisi chena

• Substrate B 7ml……………….……………………………………………………….…………kapu tsvuku

• Stop solution 7ml……….…….…………………………………………………..………yellow cap

• 20×concentrated wash solution 40ml ……………………………………………

• 2×concentrated extraction solution 50ml…………………………………………………

Sensitivity, kururama uye nemazvo

Sensitivity:0.05ppb

Detection limit

Sampuli yemafuta anodyiwa .......................................... .........................................................................0.1ppb

Nzungu................................................. .................................................. .......................0.2ppb

Zviyo................................................. .................................................. .....................0.05ppb

Kururama

Sampuli yemafuta anodyiwa .......................................... ..................................................................80±15%

Nzungu................................................. .................................................. .....................80±15%

Zviyo................................................. .................................................. .....................80±15%

Precision

Kusiyanisa coefficient ye ELISA kit iri pasi pe10%.

Cross Rate

Aflatoxin B1·················100%

Aflatoxin B2 ·····················81 .3%

Aflatoxin G1·······················62%

Aflatoxin G2 ······················22.3%

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